吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (02): 236-239.doi: 10.13481/j.1671-587x.20160209

• 基础研究 • 上一篇    下一篇

Hedgehog通路对乳腺癌细胞增殖和凋亡的作用及其对Cx32和Cx43表达的影响

孟令俊, 代恩勇, 刁建东, 毕林涛, 卢振霞   

  1. 吉林大学中日联谊医院肿瘤血液科, 吉林长春 130033
  • 收稿日期:2015-09-09 发布日期:2016-03-31
  • 通讯作者: 卢振霞,教授,硕士研究生导师(Tel:0431-84995733,E-mail:luzx2013@126.com) E-mail:luzx2013@126.com
  • 作者简介:孟令俊(1985-),男,河南省濮阳市人,主治医师,医学硕士,主要从事恶性肿瘤治疗方面的研究。
  • 基金资助:

    国家自然科学基金面上项目资助课题(30870355);吉林省科技厅科技发展计划项目资助课题(200705316)

Effects of Hedgehog on proliferationand apoptosis of breast cancer cells and its influence in Cx32 and Cx43 expressions

MENG Lingjun, DAI Enyong, DIAO Jiandong, BI Lintao, LU Zhenxia   

  1. Department of Oncology and Hematology, China-Japan Union Hospital, Jilin University, Changchun 130033, China
  • Received:2015-09-09 Published:2016-03-31

摘要:

目的: 研究Hedgehog通路对乳腺癌细胞增殖、凋亡及间隙连接蛋白32(Cx32)和间隙连接蛋白43(Cx43)表达的影响,探讨其在乳腺癌细胞增殖和转移中的作用机制。方法: 取对数生长期乳腺癌MCF-7细胞分为环巴胺组和空白对照组,环巴胺组分别以5、10、20、30和40 μmol·L-1环巴胺处理MCF-7细胞24、48和72 h,应用MTT法测定各组MCF-7细胞增殖抑制率。以0(阴性对照组)和25 μmol·L-1环巴胺处理MCF-7细胞48 h后,AnnexinⅤ/PI双染流式细胞术检测细胞凋亡率及Cx32、Cx43胞膜阳性表达率。结果: 与空白对照组比较,各剂量环巴胺组MCF-7细胞增殖抑制率明显升高(P<0.05),且随环巴胺剂量增加和作用时间延长其增殖抑制作用增强;与空白对照组比较,25 μmol·L-1环巴胺组48 h时,MCF-7细胞凋亡率明显升高(P<0.05)。与阴性对照组比较,25 μmol·L-1环巴胺组48 h时,MCF-7细胞胞膜Cx32和Cx43阳性表达率明显升高(P<0.05)。结论: Hedgehog通路可抑制乳腺癌细胞凋亡,并调节Cx43和Cx32的胞膜表达。

关键词: Hedgehog通路, 乳腺肿瘤, 环巴胺, 间隙连接蛋白

Abstract:

Objective: To study the effects of Hedgehog signal transduction pathway on the cell proliferation, apoptosis and connexin 32(Cx32) and connexin 43(Cx43) membranous distribution of breast cancer cells, and to explore its mechanism in the cell proliferation and metastasis of breast cancer.Methods: The breast cancer MCF-7 cells at logarithmic growth period were divided into cyclopamine groups and blank control groups.The MCF-7 in cyclopamine groups were treated with 5, 10, 20, 30 and 40 μmol·L-1 for 24, 48 and 72 h;MTT assay was applied to detect the inhibitory rate of proliferation of MCF-7 cells.After the MCF-7 cells were treated with 0(negative control group) and 25 μmol·L-1 cyclopamine for 48 h, flow cytometry was employed to determine the apoptotic rate and to analyze membranous distribution of Cx32 and Cx43 in the MCF-7 cells.Results: Compared with blank control group, the inhibitory rates of proliferation in cyclopamine groups were increased(P<0.05), and the inhibitory effect of proliferation was increased with the increasing of cyclopamine doses and prolongation of treatment time. After treated with 25 μmol·L-1 cyclopamine, the apoptotic rate of MCF-7 cells was higher than that in blank control group(P<0.05).The positive expression rates of Cx32 and Cx43 48 h after treatment were higher than those in negative control group(P<0.05).Conclusion: Hedgehog signal transduction pathway can inhibit the apoptosis and mediate membranous distribution of Cx32 and Cx43 in breast cancer cells.

Key words: Hedgehog pathway, breast neoplasms, cyclopamine, connexin

中图分类号: 

  • R735.3