关键词: 高活性小牛血去蛋白提取物, 酒精性肝损伤, 氧化应激

Abstract:

Objective: To observe the protective effect of deproteinized extract of calf blood(DECB) on the ethanol-induced liver injury of the mice, and to preliminaryly discuss its mechanism. Methods: Sixty healthy ICR mice were divided into control group, model group, positive drug group,low,medium and high doses of DECB groups (n=10).By intragastric administration, the mice in control group were given 20 mL·kg^-1saline solution, the mice in low, medium and high doses of DECB groups were administrated with 0.125,0.250,0.500 g·kg ^-1 DECB, and the mice in positive drug group were administrated with 0.63 g·kg ^-1Hugan Tablets;once a day for 30 d. 1 h after the last administration, except control group,the mice in other groups were administrated with one-time grant of 50% ethanol 14 mL·kg ^-1, and fasted for 16h to establish the models of acute alcohol liver injury. The endurance alcohol time and drunk time of the mice were determined,the activities of aspartate aminotransferase (ALT) and alanine transaminase (AST) activity in serum of the mice were detected, the levels of triglyceride (TG), glutathione (GSH) and malonic dialdehyde (MDA) in liver tissue were determined,and the pathological changes of liver tissue were detected. Results: Compared with model group, the drunk symptoms of the mice in different doses of DECB groups were obviously reduced, the endurance time of the mice in high dose of DECB group and positive drug group was prolonged (P < 0.05), and the drinking time was shortened (P < 0.05);the ALT and AST activities in serum in mediun and high doses of DECB groups were significantly lower than those in model group (P < 0.05).Compared with model group, the MDA and TG levels in liver tissue of the mice in medium and high doses of DECB groups and positive drug group were obviously reduced, and the GSH levels were increased(P < 0.05);compared with model group, the pathological damages of liver tissue of the mice in high dose of DECB group caused by ethanol were significantly reduced. Conclusion: DECB can improve ethanol-induced liver injury which may be related to the inhibition of hepatic oxidative stress response.

Key words: high activity deproteinized extract of calf blood, alcoholic liver injury, oxidative stress