“温阳补气”针法对EAMG大鼠神经肌肉接头处AchR蛋白表达的影响

doi:10.13481/j.1671-587x.20160202

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (02): 200-203.doi: 10.13481/j.1671-587x.20160202

“温阳补气”针法对EAMG大鼠神经肌肉接头处AchR蛋白表达的影响

王喜臣¹, 沈爱花², 胡英华¹, 王洪峰¹

1. 长春中医药大学针灸基础重点研究室, 吉林长春 130117;
2. 延边大学中医学院针灸骨伤教研室, 吉林延吉 133000

收稿日期:2015-08-31 发布日期:2016-03-31
通讯作者: 王洪峰,教授,博士研究生导师(Tel:0431-86172415,E-mail:24974606@qq.com) E-mail:24974606@qq.com
作者简介:王喜臣(1972-),男,吉林省辉南县人,副研究馆员,医学硕士,主要从事特定穴配伍规律理论和临床方面的研究。
基金资助:
国家自然科学基金资助课题(81173344);教育部博士点科研基金资助课题(20112227120006);吉林省教育厅重点项目资助课题[吉教科合字(2012第64号)]

Effect of “Warming Yang and Nourishing Qi” Acupuncture on acetylcholine receptor protein expression in neuromuscular junction of rats with EAMG

WANG Xichen¹, SHEN Aihua², HU Yinghua¹, WANG Hongfeng¹

1. Key Research Center of Basic Acupunture and Moxibustion, Changchun University of Chinese Medicine, Changchun 130117, China;
2. Department of Acupuncture, Moxibustion and Traumatology, School of Chinese Medicine, Yanbian University, Yanji 133000, China

Received:2015-08-31 Published:2016-03-31

摘要/Abstract

摘要：

目的: 探讨"温阳补气"针法对实验性自身免疫性重症肌无力(EAMG)大鼠腓肠肌神经肌肉接头(NMJ)处乙酰胆碱受体(AchR)蛋白表达水平的影响,阐明针灸治疗重症肌无力(MG)的作用机制。方法: 采用AchR α1129-145多肽片段免疫接种Lewis雌性大鼠,建立EAMG大鼠模型。在建模成功的EAMG大鼠中随机选取30只,分为模型对照组10只、药物对照组10只和针刺治疗组10只,并将同期购进的未建模的10只大鼠设为空白对照组。药物对照组大鼠予以18.5 mg·kg^-1·d^-1溴吡斯的明灌胃治疗;针刺治疗组大鼠予以"温阳补气"针法治疗;其余2组不予任何处置作为对照。记录治疗前后各组大鼠Lennon症状评分。治疗结束后,取4组大鼠腓肠肌NMJ处组织,采用Western blotting法检测各组大鼠NMJ处AchR蛋白表达水平。结果: 与空白对照组比较,建模成功后治疗前模型对照组、药物对照组和针刺治疗组大鼠Lennon症状评分明显升高(P<0.01);与模型对照组比较,经过2个疗程治疗后药物对照组和针刺治疗组大鼠Lennon症状评分均明显降低(P<0.01);与空白对照组比较,模型对照组大鼠腓肠肌NMJ处AchR蛋白表达水平明显降低(P<0.01);与模型对照组比较,治疗后针刺治疗组和药物对照组EAMG大鼠腓肠肌NMJ处AchR蛋白表达水平明显升高(P<0.01)。结论: "温阳补气"针法可以提高EAMG大鼠NMJ处AchR蛋白表达水平,发挥治疗EAMG的作用。

关键词: 实验性自身免疫性重症肌无力, 温阳补气针法, 乙酰胆碱受体蛋白

Abstract:

Objective: To study the influence of "Warming Yang and Nourishing Qi" Acupuncture in the expression levels of acetylcholine receptor protein(AchR) in gastrocnemius neuromuscular junction (NMJ) of the rats with experimental autoimmune myasthenia gravis(EAMG), and to clarify the mechanism of "Warming Yang and Nourishing Qi" Acupucture in the treatment myasthenia gravis(MG).Methods: The female Lewis rats were used to establish the EAMG rat models immuned with AchRα1 129-145 polypeptide fragments.30 EAMG rats were selected randomly and divided into model control group, drug control group, and acupuncture treatment group(n=10), and another 10 rats purchased at the same period were used as blank control group.The rats in drug control group were treated with 18.5 mg·kg^-1·d^-1 pyridostigmine by gavage.And the rats in acupuncture treatment group were treated with irritating by "Warming Yang and Nourishing Qi" Acupuncture.The rats in blank control group and model control group were not treated.The Lennon symptom scores of the rats in various groups were recorded.After treatment, the tissues of gastrocnemius NMJ of the rats were taken, and the AchR protein expression levels in NMJ of the rats in various groups were detected by Western blotting method.Results: Compared with blank control group, after successfully modelling, the Lennon symptom scores of the rats in model control group, drug control group and acupuncture treatment group before treatment were significantly increased(P<0.01).Compared with model control group, the Lennon symptom scores of the rats in drug control group and acupuncture treatment group after two treatment course were significantly increased(P<0.01).Compared with blank control group, the AchR protein expression level in gastronemus NMJ of the rats in model control group was significantly decreased(P<0.01).Compared with model control group, the AchR protein expression levels in gastronemus NMJ of the rats in acupuncture treatment group and drug control group after treatment were significantly increased(P<0.01).Conclusion: "Warming Yang and Nourishing Qi" Acupuncture has good therapeutic effecton the EAMG through increasing the NMJ AchR protein expression level of the EAMG rats.

Key words: experimental autoimmune myasthenia gravis, Warming Yang and Nourishing Qi Acupuncture, acetylcholine receptor protein

中图分类号:

R746.1

王喜臣, 沈爱花, 胡英华, 王洪峰. “温阳补气”针法对EAMG大鼠神经肌肉接头处AchR蛋白表达的影响[J]. 吉林大学学报(医学版), 2016, 42(02): 200-203.

WANG Xichen, SHEN Aihua, HU Yinghua, WANG Hongfeng. Effect of “Warming Yang and Nourishing Qi” Acupuncture on acetylcholine receptor protein expression in neuromuscular junction of rats with EAMG[J]. Journal of Jilin University Medicine Edition, 2016, 42(02): 200-203.

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“温阳补气”针法对EAMG大鼠神经肌肉接头处AchR蛋白表达的影响

Effect of “Warming Yang and Nourishing Qi” Acupuncture on acetylcholine receptor protein expression in neuromuscular junction of rats with EAMG

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