急性冠脉综合征患者内皮细胞微粒与CD4+CD25+Foxp3+调节性T细胞的关系

doi:10.13481/j.1671-587x.20160524

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (05): 963-967.doi: 10.13481/j.1671-587x.20160524

急性冠脉综合征患者内皮细胞微粒与CD4⁺CD25⁺Foxp3⁺调节性T细胞的关系

陆永光¹, 李浪², 苏强², 曾晓聪², 严华¹, 黄军章¹

1. 广西壮族自治区钦州市第二人民医院心内科, 广西钦州 535099;
2. 广西医科大学第一附属医院心内科, 广西南宁 530021

收稿日期:2015-09-15 出版日期:2016-09-28 发布日期:2016-09-29
作者简介:陆永光(1977-),男,广西壮族自治区容县人,副主任医师,医学博士,主要从事冠心病基础与临床方面的研究。
基金资助:
广西壮族自治区科技厅自然科学基金资助课题（2012GXNSFBA053113）；广西壮族自治区卫计委自筹经费资助课题（Z2011103）；广西壮族自治区钦州市科技局科技计划项目资助课题（20164419）

Relationship between endothelial microparticles and CD4⁺CD25⁺ Foxp3⁺ regulatory T cells in patients with acute coronary syndrome

LU Yongguang¹, LI Lang², SU Qiang², ZENG Xiaocong², YAN Hua¹, HUANG Junzhang¹

1. Department of Cardiology, Second People's Hospital of Qinzhou, Guangxi Zhuang Autonomous Region, Qinzhou 535099, China;
2. Department of Cardiology, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China

Received:2015-09-15 Online:2016-09-28 Published:2016-09-29

摘要/Abstract

摘要：

目的：探讨急性冠脉综合征（ACS）患者外周血内皮细胞微粒（EMPs）水平与CD4⁺CD25⁺Foxp3⁺调节性T细胞（Treg）及其细胞因子水平的关系，阐明EMPs通过影响Treg细胞分化和功能参与ACS发病过程的机制。方法：选取稳定性心绞痛患者23例（SAP组），ACS患者52例（ACS组）；以冠状动脉造影检查正常的住院患者20例作为对照组。采用流式细胞术检测各组患者外周血EMPs水平和Treg细胞百分率，实时定量PCR法检测转录因子Foxp3mRNA表达，酶联免疫吸附法检测血浆TGF-β₁水平。对EMPs与Treg细胞、Foxp3 mRNA表达水平和TGF-β1水平进行直线相关分析。结果：与对照组和SAP组比较，ACS组患者外周血中EMPs水平明显升高（P<0.01），CD4⁺CD25⁺Foxp3⁺Treg百分率、Foxp3 mRNA表达水平和血浆TGF-β₁水平明显降低（P<0.01）。EMPs与CD4⁺CD25⁺Foxp3⁺ Treg、Foxp3 mRNA表达水平和TGF-β₁水平呈负相关关系（r=-0.452，P=0.001；r=-0.466，P=0.001；r=-0.555，P=0.000）。结论：EMPs可能通过调控CD4⁺CD25⁺Foxp3⁺ Treg的分化和功能，参与冠状动脉粥样硬化的发生发展及斑块不稳定的过程。

关键词: 急性冠脉综合征, 内皮细胞微粒, 调节性T细胞, 细胞分化

Abstract:

Objective: To explore the relationships between the endothelial microparticles (EMPs) in peripheral blood and CD4⁺CD25⁺Foxp3⁺ regulatory T cells and their cytokine levels in the patients with acute coronary syndrome(ACS), and to clarify the mechanism of EMPs in the pathogenesis of ACS by affecting the Treg cell differentiation and function.Methods: Twenty-three patients with stable angina (SAP) were allocated to SAP group, and 52 patients with ACS were allocated to ACS group. Twenty individuals with normal conventional coronary angiography results were recruited as control group. The levels of EMPs and the percents of CD4⁺CD25⁺Foxp3⁺ regulatory T cells in peripheral blood of the patients in various groups were measured by flow cytometry. The expressions of Foxp3 mRNA and the plasma levels of TGF-β₁ were detected with Real-Time PCR and ELISA, respectively. Correlation analysis was performed between EMPs and regulatory T cells, Foxp3 mRNA expression level and TGF-β₁ level. Results: Compared with control group and SAP group, the level of EMPs in peripheral blood of the patients in ACS group was increased significantly, but the percentage of regulatory T cells,the Foxp3 mRNA expression level and the plasma TGF-β₁ level were decreased markedly(P<0.01). The correlation analysis showed that there were significantly negative correlation between EMPs and regulatory T cells, Foxp3 mRNA expression level and TGF-β₁ level(r=-0.452,P=0.001;r=-0.466,P=0.001;r=-0.555,P=0.000). Conclusion: EMPs may involve in the process of occurrence and development of ACS and plaque instability by regulating the differentiation and function of CD4⁺CD25⁺Foxp3⁺ regulatory T cells.

Key words: acute coronary syndrome, endothelial microparticles, regulatory T cell, cell differentiation

中图分类号:

R541.4

陆永光, 李浪, 苏强, 曾晓聪, 严华, 黄军章. 急性冠脉综合征患者内皮细胞微粒与CD4⁺CD25⁺Foxp3⁺调节性T细胞的关系[J]. 吉林大学学报(医学版), 2016, 42(05): 963-967.

LU Yongguang, LI Lang, SU Qiang, ZENG Xiaocong, YAN Hua, HUANG Junzhang. Relationship between endothelial microparticles and CD4⁺CD25⁺ Foxp3⁺ regulatory T cells in patients with acute coronary syndrome[J]. Journal of Jilin University Medicine Edition, 2016, 42(05): 963-967.

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急性冠脉综合征患者内皮细胞微粒与CD4⁺CD25⁺Foxp3⁺调节性T细胞的关系

Relationship between endothelial microparticles and CD4⁺CD25⁺ Foxp3⁺ regulatory T cells in patients with acute coronary syndrome

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急性冠脉综合征患者内皮细胞微粒与CD4+CD25+Foxp3+调节性T细胞的关系

Relationship between endothelial microparticles and CD4+CD25+ Foxp3+ regulatory T cells in patients with acute coronary syndrome

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急性冠脉综合征患者内皮细胞微粒与CD4⁺CD25⁺Foxp3⁺调节性T细胞的关系

Relationship between endothelial microparticles and CD4⁺CD25⁺ Foxp3⁺ regulatory T cells in patients with acute coronary syndrome