HPK1过表达对乳腺癌MCF-7和MDA-MB-231细胞增殖和凋亡的影响及其机制

doi:10.13481/j.1671-587x.20170510

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (05): 910-917.doi: 10.13481/j.1671-587x.20170510

HPK1过表达对乳腺癌MCF-7和MDA-MB-231细胞增殖和凋亡的影响及其机制

王娇娇¹, 范智蕊², 李砺锋³, 丁显飞⁴, 周学良², 赵杰⁵, 王留兴²

1. 郑州大学第一附属医院超声科, 河南郑州 450052;
2. 郑州大学第一附属医院肿瘤科, 河南郑州 450052;
3. 郑州大学第一附属医院生物细胞治疗中心, 河南郑州 450052;
4. 郑州大学第一附属医院综合ICU, 河南郑州 450052;
5. 郑州大学第一附属医院药学部, 河南郑州 450052

收稿日期:2017-05-20 出版日期:2017-09-28 发布日期:2017-09-29
通讯作者: 王留兴,教授,博士研究生导师(Tel:0371-66913289,E-mail:lxwang2006@126.com) E-mail:lxwang2006@126.com
作者简介:王娇娇(1983-),女,河南省安阳县人,主治医师,在读医学博士,主要从事乳腺癌的超声诊断及发病机制方面的研究。
基金资助:
河南省科技厅科技惠民计划专项基金资助课题（2013GS410101）；河南省科技厅重大科技专项基金资助课题（151100310800）

Influence of HPK1 overexpression in proliferation and apoptosis of breast cancer MCF-7 and MDA-MB-231 cells and its mechanism

WANG Jiaojiao¹, FAN Zhirui², LI Lifeng³, DING Xianfei⁴, ZHOU Xueliang², ZHAO Jie⁵, WANG Liuxing²

1. Department of Ultrasound, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;
2. Department of Oncology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;
3. Department of Biological Cell Therapy Center, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;
4. Department of General ICU, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;
5. Department of Pharmacy, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China

Received:2017-05-20 Online:2017-09-28 Published:2017-09-29

摘要/Abstract

摘要： 目的：构建造血祖细胞激酶1（HPK1）慢病毒载体，探讨HPK1过表达对乳腺癌MCF-7和MDA-MB-231细胞增殖和凋亡的作用，阐明其可能的机制。方法：通过过表达HPK1的慢病毒感染细胞，获得稳定表达HPK1的细胞系（MCF-7-HPK1和MDA-MB-231-HPK1），将2个细胞系分别分为3组，即空白组、对照组（空载体组）和过表达组；分别采用RT-PCR法和Western blotting法检测乳腺癌细胞中HPK1 mRNA和蛋白表达水平，MTT法检测细胞增殖率，流式细胞术检测细胞周期和凋亡率，Transwell法分析细胞的迁移能力，Western blotting法检测caspase 3、PTEN、MMP-9、MMP-2、Ki-67和HPK1蛋白表达水平。结果：与空白组和对照组比较，乳腺癌MCF-7和MDA-MB-231细胞过表达组中HPK1 mRNA和蛋白表达水平升高（P < 0.05），细胞增殖率明显降低（P < 0.05），细胞凋亡率明显升高（P < 0.05），穿越Matrigel的细胞数明显减少（P < 0.05），MCF-7细胞周期阻断在G₁期；过表达组细胞中caspase 3、PTEN蛋白表达水平上调（P < 0.05），MMP-9、MMP-2、Ki-67蛋白表达水平降低（P < 0.05）。结论：HPK1过表达可抑制乳腺癌细胞MCF-7和MDA-MB-231的增殖及迁移并诱导凋亡，其可能与caspase 3、PTEN蛋白的表达上调及MMP-9、MMP-2和Ki-67蛋白的表达降低有关。

关键词: 造血祖细胞激酶1, 细胞增殖, 乳腺癌细胞株, 细胞凋亡

Abstract: Objective: To investigate the effects of hematopoietic progenitor kinase 1 (HPK1) overexpression by construction of lentiviral vector on the proliferation and apoptosis of breast cancer MCF-7 and MDA-MB-231 cells, and to elucidate its possible mechanism. Methods: The cells were infected with the lentivirus overxpressing HPK1,and the MCF-7-HPK1 and MDA-MB-231-HPK1 cell lines were stably expressed HPK1;each cell line was divided into three experimental groups:blank group (untreated), control group(empty vector) and HPK1-overexpression group. The expression levels of HPK1 mRNA and protein in breast cancer cells in each group were detected by RT-PCR and Western blotting methods, respectively. The cell proliferation rate was detected by MTT assay. The cell cycle and apoptotic rate were detected by flow cytometry. Transwell assay was used to analyze the cell migration ability. Western blotting method was used to measure the expression levels of caspase 3, PTEN, MMP-9,MMP-2, Ki-67and HPK1 proteins. Results: Compared with blank groups and control groups, the expression levels of HPK1 mRNA and protein in the both cell lines in HPK1 overexpression groups were significantly up-regulated(P<0.05), the proliferation rates were significantly decreased(P<0.05) and the apoptotic rates were significantly increased(P<0.05), the number of cells crossing matrigel was significantly reduced(P<0.05), the cell cycle of MCF-7 was blocked in G1 phase(P<0.05), the expression levels of caspase 3 and PTEN proteins in HPK1 overexpression group were significantly increased(P<0.05), and the expression levels of MMP-2 and MMP-9 proteins were significantly decreased (P<0.05). Conclusion: HPK1 overexpression can inhibit the proliferation and migration of MCF-7 and MDA-MB-231 cells and induce apoptosis, which may be related to the up-regulation of caspase 3 and PTEN and down-regulation of MMP-9, MMP-2 and Ki-67.

Key words: hematopoietic progenitor kinase 1, breast cell lines, cell proliferation, apoptosis

中图分类号:

R737.9

王娇娇, 范智蕊, 李砺锋, 丁显飞, 周学良, 赵杰, 王留兴. HPK1过表达对乳腺癌MCF-7和MDA-MB-231细胞增殖和凋亡的影响及其机制[J]. 吉林大学学报(医学版), 2017, 43(05): 910-917.

WANG Jiaojiao, FAN Zhirui, LI Lifeng, DING Xianfei, ZHOU Xueliang, ZHAO Jie, WANG Liuxing. Influence of HPK1 overexpression in proliferation and apoptosis of breast cancer MCF-7 and MDA-MB-231 cells and its mechanism[J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 910-917.

参考文献

[1] Siegel RL, Miller KD, Jemal A. Cancer Statistics, 2017[J]. CA Cancer J Clin, 2017, 67(1):7-30.
[2] 丁显飞, 周学良, 豆萌萌, 等. 哺乳动物雷帕霉素靶蛋白的表达及其与乳腺癌预后关系的Meta分析[J]. 吉林大学学报:医学版,2016, 42(4):783-788.
[3] Ling P, Yao Z, Meyer CF, et al. Interaction of hematopoietic progenitor kinase 1 with adapter proteins Crk and CrkL leads to synergistic activation of c-Jun N-terminal kinase[J]. Mol Cell Biol, 1999, 19(2):1359-1368.
[4] Chuang HC, Wang X, Tan TH. MAP4K family kinases in immunity and inflammation[J]. Adv Immunol, 2016, 129:277-314.
[5] Sawasdikosol S, Zha R, Yang B, et al. HPK1 as a novel target for cancer immunotherapy[J]. Immunol Res, 2012, 54(1-3):262-265.
[6] Wang H, Song X, Logsdon C, et al. Proteasome-mediated degradation and functions of hematopoietic progenitor kinase 1 in pancreatic cancer[J]. Cancer Res, 2009, 69(3):1063-1070.
[7] Alzabin S, Pyarajan S, Yee H, et al. Hematopoietic progenitor kinase 1 is a critical component of prostaglandin E2-mediated suppression of the anti-tumor immune response[J]. Cancer Immunol Immunother, 2010, 59(3):419-429.
[8] Li Z, Park HR, Shi Z, et al. Pro-oncogenic function of HIP-55/Drebrin-like (DBNL) through Ser269/Thr291-phospho-sensor motifs[J]. Oncotarget, 2014, 5(10):3197-3209.
[9] Wang Y, Luo H, Li Y, et al. hsa-miR-96 up-regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas[J]. Mol Med Rep, 2012, 5(1):260-265.
[10] 王娇娇, 宋丽杰, 杨森, 等. 造血祖细胞激酶1在雌激素受体阳性乳腺癌中的表达及其与预后的关系[J]. 中华实验外科杂志, 2016, 33(8):2022-2025.
[11] Tavangar F, Sepehri H, Saghaeian Jazi M, et al. Amphotericin B potentiates the anticancer activity of doxorubicin on the MCF-7 breast cancer cells[J]. J Chem Biol, 2017, 10(3):143-150.
[12] van der Heijden AG, Mengual L, Lozano JJ, et al. A five-gene expression signature to predict progression in T1G3 bladder cancer[J]. Eur J Cancer, 2016, 64:127-136.
[13] Lin M, Zhang Y, Li A, et al. High-throughput RNAi screening of human kinases identifies predictors of clinical outcome in colorectal cancer patients treated with oxaliplatin[J]. Oncotarget, 2015, 6(18):16774-16785.
[14] 孟凡荣, 陈琛, 万海粟, 等. 慢病毒载体及其研究进展[J]. 中国肺癌杂志, 2014(12):870-876.
[15] Yan F, He Q, Hu X, et al. Direct regulation of caspase-3 by the transcription factor AP-2 is involved in aspirininduced apoptosis in MDAMB453 breast cancer cells[J]. Mol Med Rep, 2013, 7(3):909-914.
[16] Yelken Bö, Balci T, Süslüer SY, et al. The effect of tomatine on metastasis related matrix metalloproteinase (MMP) activities in breast cancer cell model[J]. Gene, 2017, 627:408-411.
[17] Harvey J, Thomas C, Wood B, et al. Practical issues concerning the implementation of Ki-67 proliferative index measurement in breast cancer reporting[J]. Pathology, 2015, 47(1):13-20.

HPK1过表达对乳腺癌MCF-7和MDA-MB-231细胞增殖和凋亡的影响及其机制

Influence of HPK1 overexpression in proliferation and apoptosis of breast cancer MCF-7 and MDA-MB-231 cells and its mechanism

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