骨膜蛋白在慢性牙周炎患者牙龈组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20190327

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (03): 634-638.doi: 10.13481/j.1671-587x.20190327

• 临床研究 • 上一篇

骨膜蛋白在慢性牙周炎患者牙龈组织中的表达及其临床意义

金权^1,2, 郑义¹, 许立硕¹, 黄玉¹, 马宁¹, 张莉³

1. 吉林大学口腔医院牙周科, 吉林长春 130021;
2. 吉林省牙发育及颌骨重塑与再生重点实验室, 吉林长春 130021;
3. 吉林大学口腔医院急诊科, 吉林长春 130021

收稿日期:2019-01-18 发布日期:2019-06-05
通讯作者: 马宁,教授,主任医师,硕士研究生导师(Tel:0431-88796039,E-mail:maningbsh76@sina.com);张莉,副教授,副主任医师,硕士研究生导师(Tel:0431-85579359,E-mail:zlamy1009@sina.com) E-mail:maningbsh76@sina.com;zlamy1009@sina.com
作者简介:金权(1990-),男,吉林省龙井市人,在读医学硕士,主要从事牙周病诊治方面的研究。
基金资助:
吉林省卫计委适宜技术推广项目资助课题（2017S026）

Expression of periostin in gingival tissue of patients with chronic periodontitis and its clinical significance

JIN Quan^1,2, ZHENG Yi¹, XU Lishuo¹, HUANG Yu¹, MA Ning¹, ZHANG Li³

1. Department of Periodontics, Stomatology Hospital, Jilin University, Changchun 130021, China;
2. Key Laboratory of Tooth Development and Jaw Bone Remodeling and Regeneration, Jilin Province, Changchun 130021, China;
3. Department of Emergency, Stomatology Hospital, Jilin University, Changchun 130021, China

Received:2019-01-18 Published:2019-06-05

摘要/Abstract

摘要： 目的：检测慢性牙周炎患者牙龈组织中骨膜蛋白、基质金属蛋白酶8（MMP-8）和基质金属蛋白酶组织抑制剂2（TIMP-2）的表达情况，探讨骨膜蛋白在慢性牙周炎发生发展中的可能作用及其相关生物学机制。方法：选择23例正常牙龈组织（对照组）和19例炎症牙龈组织（病例组）。采用免疫组织化学SP法检测2组患者牙龈组织中骨膜蛋白、MMP-8和TIMP-2表达情况，采用Spearman分析法检测2组患者牙龈组织中骨膜蛋白表达与MMP-8和TIMP-2表达的相关性。结果：病例组患者牙龈组织中骨膜蛋白阳性表达率低于对照组（P<0.05）。相关性分析，对照组患者牙龈组织中骨膜蛋白表达与MMP-8表达呈负相关关系（r=-0.51，P<0.05），骨膜蛋白表达与TIMP-2表达呈正相关关系（r=0.50，P<0.05）；病例组患者牙龈组织中骨膜蛋白表达与MMP-8表达呈负相关关系（r=-0.482，P<0.05），骨膜蛋白表达与TIMP-2表达呈正相关关系（r=0.655，P<0.05）。结论：骨膜蛋白可以作为维持牙龈组织结构稳定性的初步指标，成为牙周组织再生方面的研究靶点。

关键词: 慢性牙周炎, 骨膜蛋白, 基质金属蛋白酶8, 基质金属蛋白酶组织抑制剂2

Abstract: Objective:To detect the expressions of periostin, matrix metalloproteinase-8 (MMP-8)and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2)in gingival tissue of the patients with chronic periodontitis,and to explore the possible role of periostin in the occurrence and development of chronic periodontitis and its related biological mechanism. Methods:A total of 23 normal gingival tissues(control group) and 19 inflammatory gingival tissues (case group)were selected. The expressions of periostin, MMP-8 and TIMP-2 in gingival tissue of the patients in two groups were detected by immunohistochemical SP method.The correlation between the expressions of periositn and MMP-8 and TIMP-2 in gingival tissue of the patients in two groups were detected by Spearman analysis. Results:The positive expression rate of periostin in gingival tissue of the patients in case group was lower than that in control group (P<0.05). The correlative analysis results demonstrated that the expression of periostin had negative relationship with the expression of MMP-8 (r=-0.51, P<0.05) in control group, and positive relationship with the expression of TIMP-2 (r=0.50, P<0.05);in case group, the expression of periostin had negative relationship with the expression of MMP-8 (r=-0.482, P<0.05), and positive relationship with the expression of TIMP-2 (r=0.655, P<0.05). Conclusion:Periostin can be used as a preliminary indicator for maintaining the structural stability of gingival tissue, and become a research target for periodontal tissue regeneration.

Key words: chronic periodontitis, periostin, matrix metalloproteinase-8, tissue inhibitor of matrix metalloproteinase-2

中图分类号:

R781.4

金权, 郑义, 许立硕, 黄玉, 马宁, 张莉. 骨膜蛋白在慢性牙周炎患者牙龈组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2019, 45(03): 634-638.

JIN Quan, ZHENG Yi, XU Lishuo, HUANG Yu, MA Ning, ZHANG Li. Expression of periostin in gingival tissue of patients with chronic periodontitis and its clinical significance[J]. Journal of Jilin University(Medicine Edition), 2019, 45(03): 634-638.

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骨膜蛋白在慢性牙周炎患者牙龈组织中的表达及其临床意义

Expression of periostin in gingival tissue of patients with chronic periodontitis and its clinical significance

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