不同周龄小鼠耳蜗组织中Otos的表达及其意义

doi:10.13481/j.1671-587x.20190618

摘要/Abstract

摘要： 目的：观察Otos在不同周龄年龄相关性听力损失（AHL）小鼠耳蜗组织中的表达和分布，探讨其与AHL的关系。方法：40只SPF级C57BL/6Cnc小鼠按鼠龄分成4、16、32和48周龄组，每组10只。各组小鼠行听性脑干反应（ABR）检查后取出耳蜗，免疫荧光法检测各组小鼠耳蜗组织中Otospiralin蛋白的表达量和分布，qRT-PCR法检测各组小鼠耳蜗组织中Otos mRNA表达水平，Western blotting法检测各组小鼠耳蜗组织中Otospiralin蛋白表达水平。结果：Otospiralin蛋白在小鼠耳蜗组织中主要表达于螺旋韧带且在5种纤维细胞中均有表达；与4周龄组比较，16、32和48周龄小鼠耳蜗组织中Otospiralin蛋白表达量明显降低（P<0.01）。与4周龄组比较，16、32和48周龄组小鼠ABR平均阈值均明显升高（P<0.01），耳蜗组织中Otos mRNA和Otospiralin蛋白表达水平均明显降低（P<0.01）；与16周龄组比较，32和48周龄组小鼠ABR平均阈值均明显升高（P<0.01），耳蜗组织中Otos mRNA和Otospiralin蛋白表达水平均明显降低（P<0.01）；与32周龄组比较，48周龄组小鼠ABR平均阈值明显升高（P<0.01），耳蜗组织中Otos mRNA和Otospiralin蛋白表达水平均明显降低（P<0.01）。结论：随着AHL小鼠鼠龄的增加其听觉功能逐渐减退，其耳蜗组织中Otos mRNA和Otospiralin蛋白表达水平也逐渐减少。Otos的这种改变可能参与AHL的发生发展。

关键词: 听性脑干反应, Otos, 年龄相关性听力损失, 老年性聋, 螺旋韧带

Abstract: Objective: To observe the expressions and distribution of Otos in cochlea tissue of the mice with age-related hearing loss (AHL) at different ages,and to explore its relationship with AHL. Methods: A total of 40 SPF C57BL/6Cnc mice were divided into 4-week-old group,16-week-old group,32-week-old group and 48-week-old group,and there were 10 mice in each group. The auditory brainstem response (ABR) was performed in the mice in various groups,then after the cochlea was removed,the expressions and distribution of Otospiralin protein in cochlea tissue of the mice in various groups were detected by immunofluorescence;the expression levels of Otos mRNA in cochlea tissue of the mice in various groups were examined by qRT-PCR method,and the expression levels of Otospiralin protein in cochlea tissue of the mice in various groups were detected by Western blotting method. Results: The Otospiralin proein was mainly expressed in the spiral ligament of the cochlea tissue of the mice and was expressed in all five fibrocytes;compared with 4-week-old group,the expression amounts of Otospiralin protein in cochlea tissue of the mice in 16-,32-,and 48-week-old groups were significantly increased(P<0.01).Compared with 4-week-old group,the mean ABR thresholds of the mice in 16-,32- and 48-week-old groups were significantly increased (P<0.01),and the expression levels of Otos mRNA and Otospiralin protein in cochlea tissue of the mice were significantly decreased (P<0.01);compared with 16-week-old group,the mean ABR thresholds of the mice in 32- and 48-week-old groups were significantly increased (P<0.01),and the expression levels of Otos mRNA and Otospiralin protein in cochlea tissue of the mice were significantly decreased (P<0.01); compared with 32-week-old group,the mean ABR threshold of the mice in 48-week-old group was significantly increased (P<0.01),and the expression levels of Otos mRNA and Otospiralin protein in cochlea tissue of the mice were significantly decreased (P<0.01). Conclusion: With the increasing of age,the auditory function of AHL mice is declined gradually,and the expression levels of Otos mRNA and Otospiralin protein are decreased gradually.The changes of Otos may be involved in the occurrence and development of AHL.

Key words: auditory brainstem response, Otos, age-related hearing loss, presbycusis, spiral ligament

中图分类号:

R764.43

史素楠, 刘浩, 宫亮. 不同周龄小鼠耳蜗组织中Otos的表达及其意义[J]. 吉林大学学报(医学版), 2019, 45(06): 1299-1304.

SHI Sunan, LIU Hao, GONG Liang. Expressions of Otos in cochlear tissue of mice at different ages and their significances[J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1299-1304.

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