乳双歧杆菌M8对免疫抑制模型大鼠免疫功能的影响

doi:10.13481/j.1671-587x.20200325

摘要/Abstract

摘要： 目的：探讨给予乳双歧杆菌M8后免疫抑制模型大鼠淋巴细胞亚群百分比、T淋巴细胞转化增殖活性、血清免疫球蛋白和细胞因子表达水平，阐明乳双歧杆菌M8对免疫抑制模型大鼠免疫功能的影响。方法：50只Wistar大鼠随机分为空白对照组、模型对照组、低剂量乳双歧杆菌M8组、中剂量乳双歧杆菌M8组和高剂量乳双歧杆菌M8组，每组10只。各组大鼠适应性灌服益生菌乳双歧杆菌M8 6 d，第7天开始除空白对照组外，其余4组大鼠每日给予环磷酰胺（CTX），连续3 d，构建大鼠免疫抑制模型，造模完成后低、中和高剂量乳双歧杆菌M8组大鼠给予0.01、0.10和1.00 g乳双歧杆菌M8，空白对照组和模型对照组大鼠灌服等体积无菌生理盐水。流式细胞术检测各组大鼠全血淋巴细胞亚群（CD3+T淋巴细胞、CD4+T淋巴细胞和CD8+T淋巴细胞、B淋巴细胞和NK细胞）百分比，CCK-8法检测各组大鼠T淋巴细胞转化增殖活性，ELISA法检测各组大鼠血清免疫球蛋白A （IgA）、免疫球蛋白G （IgG）、白细胞介素2（IL-2）、白细胞介素6（IL-6）、干扰素γ（IFN-γ）和肿瘤坏死因子α（TNF-α）水平。结果：与空白对照组比较，模型对照组大鼠全血CD3+T淋巴细胞、CD4+T淋巴细胞、CD8+T淋巴细胞、B淋巴细胞和NK细胞百分比明显降低（P<0.05）；与模型对照组比较，高剂量乳双歧杆菌M8组大鼠全血CD3+T淋巴细胞、CD4+T淋巴细胞和CD8+T淋巴细胞百分比明显升高（P<0.05或P<0.01），B淋巴细胞和NK细胞百分比明显升高（P<0.01）。与空白对照组比较，模型对照组大鼠T淋巴细胞转化增殖活性降低（P<0.01）；与模型对照组比较，中和高剂量乳双歧杆菌M8组大鼠T淋巴细胞转化增殖活性升高（P<0.01）。与空白对照组比较，模型对照组大鼠血清IgA、IgG、IL-2、IL-6、IFN-γ和TNF-α水平降低（P<0.01）；与模型对照组比较，中和高剂量乳双歧杆菌M8组大鼠血清IgA、IgG、IL-2、IL-6、IFN-γ和TNF-α水平升高（P<0.05或P<0.01）。结论：灌服乳双歧杆菌M8可以提高免疫抑制模型大鼠的特异性和非特异性免疫应答调控能力，乳双歧杆菌M8对免疫抑制大鼠免疫调节有一定促进作用。

关键词: 乳双歧杆菌M8, 环磷酰胺, 淋巴细胞, 转化增殖, 淋巴细胞亚群, 细胞因子

Abstract: Objective: To investigate the percentages of lymphocyte subsets, the transformation and proliferation activities of T lymphocytes, the expression levels of serum immunoglobulin and cytokines in the immunosuppressive model rats after given Bifidobacterium lactis M8, and to elucidate the effect of Bifidobacterium lactis M8 on the immune function of the immunosuppressive model rats. Methods: Fifty Wistar rats were randomly divided into blank control group, model control group, low dose of Bifidobacterium lactis M8 group, middle dose of Bifidobacterium lactis M8 group, and high dose of Bifidobacterium lactis M8 group, and there were 10 rats in each group.The rats in each group were administrated with probiotic bifidobacterium lactis M8 for 6 d.From the 7th day, except for blank control group, the rats in the other groups were given cyclophosphamide (CTX) for 3 d to construct the immunosuppressive models; the rats in low, middle, and high doses of Bifidobacterium lactis M8 groups were given 0.01, 0.10 and 1.00 g bifidobacterium lactis M8, and the rats in blank control group and model control group were given sterile saline with the same volume. The percentages of lymphocyte subsets (CD3+ T lymphocytes, CD4+ T lymphocytes, CD8+ T lymphocytes, B lymphocytes and NK cells) in whole blood of the rats in various groups were detected by flow cytometry, the transformation and proliferation activities of T lymphocytes of the rats in various groups were detected by CCK-8 method, the levels of serum immunoglobulin A (IGA), immunoglobulin G (IgG), interleukin-2(IL-2), interleukin-6(IL-6), interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) of the rats in various groups were detected by ELISA method. Results: Compared with blank control group, the percentages of CD3+ T lymphocytes, CD4+ T lymphocytes, CD8+ T lymphocytes, B lymphocytes and NK cells in whole blood of the rats inmodel control group were decreased significantly (P<0.05); compared with model control group, the percentages of CD3+ T lymphocytes, CD4+ T lymphocytes and CD8+ T lymphocytes in whole blood of the rats in high dose of Bifidobacterium lactis M8 group were increased significantly (P<0.05 or P<0.01),and the percentages of B lymphocytes and NK cells were increased significantly (P<0.01).Compared with blank control group, the transformation and proliferation activities of T lymphocytes of the rats in model control group was decreased (P<0.01); compared with model control group, the transformation and proliferation activities of T lymphocytes of the rats in middle and high doses of Bifidobacterium lactis M8 group were increased (P<0.01). Compared with blank control group, the levels of serum IgA, IgG, IL-2, IL-6, IFN-γ and TNF -α of the rats in model control group were decreased (P<0.01); compared with model control group, the levels of serum IgA, IgG, IL-2, IL-6, IFN-γ and TNF-α of the rats in middle and high doses of Bifidobacterium lactis M8 group were increased (P<0.05 or P<0.01). Conclusion: Perfusion of Bifidobacterium lactis M8 can improve the specific and non-specific immune response regulation ability of the immunosuppressed model rats. Bifidobacterium lactis M8 has a promotion effect on the immune regulation of the immunosuppressed rats.

Key words: Bifidobacterium lactis M8, cyclophosphamide, lymphocyte, transformation and proliferation, lymphocyte subset, cytokine

中图分类号:

Q939.91

翟云, 王雅菲, 托娅. 乳双歧杆菌M8对免疫抑制模型大鼠免疫功能的影响[J]. 吉林大学学报(医学版), 2020, 46(03): 582-588.

ZHAI Yun, WANG Yafei, TUO Ya. Effect of Bifidobacterium lactis M8 on immune function of immunosuppressive model rats[J]. Journal of Jilin University(Medicine Edition), 2020, 46(03): 582-588.

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