黄芪联合胰岛素对糖尿病大鼠胰岛素抵抗的作用及其机制

doi:10.7694/jldxyxb20130212

吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (2): 246-250.doi: 10.7694/jldxyxb20130212

黄芪联合胰岛素对糖尿病大鼠胰岛素抵抗的作用及其机制

李建会¹，李云²，任治兴¹，高影³

1.吉林大学公共卫生学院卫生毒理学教研室，吉林长春 130021；2.吉林大学中日联谊医院眼科，吉林长春 130033；3.吉林大学第一医院内分泌科，吉林长春 130021

收稿日期:2012-12-17 出版日期:2013-03-28 发布日期:2013-03-26
通讯作者: 高影（Tel:0431-88782557，E-mail: gaoyingcc@sina.com.cn） E-mail:gaoyingcc@sina.com.cn
作者简介:李建会（1985-），男，河北省承德市人，在读医学硕士，主要从事糖尿病及并其发症诊治的研究。
基金资助:
吉林省科技厅科研基金资助课题（2009011-1）

Effect of astragalus combined with insulin on insulin resistance in diabetic rats

LI Jian-hui¹,LI Yun²,REN Zhi-xing¹,GAO Ying³

1. Department of Health Toxicology,School of Public Health,Jilin University,Changchun 130021,China;2. Department of Ophthalmology,China-Japan Union Hospital,Jilin University,Changchun 130033,China;3. Department of Endocrinology,First Hospital,Jilin University,Changchun 130021,China

Received:2012-12-17 Online:2013-03-28 Published:2013-03-26

摘要/Abstract

摘要： ［摘要］目的:探讨黄芪联合胰岛素对糖尿病大鼠胰岛素抵抗的作用，并阐明其作用机制。方法:链脲佐菌素（STZ）诱导糖尿病大鼠模型，将诱导成功的糖尿病大鼠随机分为模型组(DM)、黄芪组(RA)、黄芪联合胰岛素组（RA+Ins）和胰岛素组(Ins)，同时设立正常大鼠为对照组（Control）。对照组和DM组大鼠无任何处理因素；RA组大鼠给予每日黄芪灌胃；Ins组大鼠每日胰岛素注射；RA+Ins组大鼠给予黄芪灌胃和胰岛素注射。8周后测定大鼠空腹血糖(FBG)值和空腹胰岛素(FINS)水平。腹主动脉取血，检测大鼠血清丙二醛（MDA）含量、超氧化物歧化酶（SOD）活力、肿瘤坏死因子α（TNF-α）含量及大鼠骨骼肌胰岛素受体底物-1（IRS-1）和p38MAPK的表达水平。结果：DM组、RA组、Ins组和RA+Ins组大鼠FBG水平显著高于对照组（P<0.05），RA组、Ins组和RA+Ins组大鼠FBG水平显著低于DM组（P<0.05），Ins组和RA+Ins组大鼠FBG水平显著低于RA组（P<0.05），并且RA+Ins组大鼠FBG水平高于Ins组（P<0.05）。Ins组、RA+Ins组大鼠FINS水平显著高于对照组、DM组和RA组（P<0.01），RA+Ins组大鼠FINS水平显著低于Ins组（P<0.01）。DM组、RA组、Ins组和RA+Ins组大鼠血清MDA含量显著高于对照组（P<0.05），RA组、Ins组和RA+Ins组大鼠血清MDA含量明显低于DM组（P<0.05)，RA组和Ins组大鼠血清MDA含量明显高于RA+Ins组（P<0.05）。DM组、RA组、Ins组和RA+Ins组大鼠SOD活力显著低于对照组（P<0.05），RA组、Ins组和RA+Ins组大鼠血清SOD活力显著高于DM组（P<0.05)，RA组和Ins组大鼠血清SOD活力显著低于RA+Ins组（P<0.05）。DM组、RA组、Ins组和RA+Ins组大鼠血清TNF-α含量显著高于对照组（P<0.05），RA组、Ins组和RA+Ins组大鼠血清TNF-α含量明显低于DM组（P<0.05），RA+Ins组大鼠血清TNF-α含量明显低于RA组和Ins组（P<0.05)。DM组、RA组、Ins组和RA+Ins组大鼠IRS-1表达低于对照组（P<0.05）,RA+Ins组大鼠IRS-1表达高于DM组、RA组和Ins组（P<0.05)。DM组、RA组、Ins组和RA+Ins组大鼠p38MAPK表达高于对照组（P<0.05),RA+Ins组大鼠p38MAPK表达低于DM组、RA组和Ins组（P<0.05)。结论：黄芪联合胰岛素明显改善了糖尿病大鼠氧化应激及胰岛素抵抗状态，并且疗效优于单纯胰岛素的应用。

关键词: 黄芪, 糖尿病, 胰岛素抵抗, 胰岛素受体底物-1, p38 MAPK

Abstract: Abstract:Objective To explore the effect of astragalus combined with insulin on insulin resistance in diabetic rats and to clarify the action mechanism.Methods The diabetic rat models induced by STZ were randomly divided into diabetic model(DM) group,astragalus(RA) group,insulin(Ins) group and astragalus combined with insulin(RA+Ins) group.At the same time the normal rats were used as control group.After treatment for 8 weeks,the levels of fasting blood glucose(FBG) and fasting insulin(FINS) of rats were measured,and the contents of MDA and TNF-α in serum were detected,and the levels of insulin receptor substrate-1(IRS-1) and p38MAPK in skeletal muscle were examined by Western blotting.Results Compared with control group,the levels of FBG of rats in DM，RA，Ins and RA+Ins groups were significantly increased (P<0.05)，and the FBG levels in RA,Ins and RA+Ins groups were significantly decreased compared with DM group(P<0.05);the FBG level in RA+Ins group was significantly decreased compared with RA and Ins groups(P<0.05).Compared with DM,RA and control groups,the levels of FINS in Ins and RA+Ins groups were increased(P<0.05),and the FINS level in RA+Ins group was decreased compared with Ins group(P<0.05).Compared with control group,the levels of MDA and TNF-α in DM,RA,Ins and RA+Ins groups were significantly increased(P<0.05),and they were lower in RA,Ins and RA+Ins groups compared with DM group(P<0.05);compared with RA and Ins groups,the MDA and TNF-α in RA+Ins were decreased(P<0.05).The activities of SOD in DM, RA,Ins and RA+Ins groups were significantly decreased compared with control group(P<0.05),and they were higher in RA,Ins and RA+Ins groups compared with DM group(P<0.05);compared with Ins and RA groups,it was increased in RA+Ins group(P<0.05).The expression levels of IRS-1 in DM,RA and RA+Ins groups were decreased compared with control group(P<0.05),but it was higher in RA+Ins than DM,RA and Ins groups(P<0.05).The expression levels of p38MAPK in DM,RA,Ins and RA+Ins groups were increased compared with control group(P<0.05),but it was lower in RA+Ins group than DM,RA and Ins groups(P<0.05).Conclusion Astragalus combined with insulin significantly improve the oxidative stress and insulin resistance in diabetic rats,and its efficacy is better than insulin alone.

Key words: astragalus, diabetes mellitus, insulin resistance, insulin receptor substrate-1, p38 MAPK

中图分类号:

R587.1

李建会，李云，任治兴，高影. 黄芪联合胰岛素对糖尿病大鼠胰岛素抵抗的作用及其机制[J]. 吉林大学学报(医学版), 2013, 39(2): 246-250.

LI Jian-hui,LI Yun,REN Zhi-xing,GAO Ying . Effect of astragalus combined with insulin on insulin resistance in diabetic rats[J]. Journal of Jilin University Medicine Edition, 2013, 39(2): 246-250.

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黄芪联合胰岛素对糖尿病大鼠胰岛素抵抗的作用及其机制

Effect of astragalus combined with insulin on insulin resistance in diabetic rats

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