孕期至幼年期低水平铅暴露仔鼠海马锌离子平衡的变化及其发育期海马神经元的损伤

doi:10.7694/jldxyxb20130320

吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (3): 521-528.doi: 10.7694/jldxyxb20130320

孕期至幼年期低水平铅暴露仔鼠海马锌离子平衡的变化及其发育期海马神经元的损伤

李海波，刘行*，孙美花,梁建民

吉林大学第一医院儿科门诊，吉林长春 130021

收稿日期:2012-06-07 出版日期:2013-05-28 发布日期:2013-07-01
通讯作者: 梁建民 (Tel：0431-88782493，E-mail：jackliangjm@163.com) E-mail:jackliangjm@163.com
作者简介:李海波（1961-），男，吉林省长春市人，教授，医学硕士，主要从事小儿神经系统疾病的基础及临床研究。
基金资助:
吉林省科技厅高技术产业发展专项基金资助课题(200905139)；吉林省发展和改革委员会基金资助课题

Change of zinc balance in hippocampus of pup rats after lead exposure from pregnancy to juvenile stage and hippocampal neuron injury in development stage

LI Hai-bo,LIU Xing*,SUN Mei-hua,LIANG Jian-min

Department of Pediatrics,First Hospital,Jilin University,Changchun 130021,China

Received:2012-06-07 Online:2013-05-28 Published:2013-07-01

摘要/Abstract

摘要： 目的:探讨孕期至幼年期铅暴露对仔鼠发育期海马神经元的损伤及其与Zn2+平衡的相关机制。方法：25只母鼠随机分为对照组母鼠（n=10）和铅暴露组母鼠（n=15）。从受孕当天开始至仔鼠生后7、14和21　d时间点分别将对照组和铅暴露组仔鼠随机各分为3组(P7、P14和P21组)，每小组仔鼠8只，铅暴露组母鼠按上述时间段给予0.2%醋酸铅饮水，对照组母鼠正常饮水。在仔鼠生后上述时间点分别测定仔鼠血铅和脑铅含量，Timm’s染色观察海马苔藓纤维(MF)，并采用免疫组织化学法检测锌离子转运体-1（ZnT-1）的表达水平。结果：铅暴露组仔鼠血铅和脑铅含量显著高于对照组（P<0.01）。铅暴露组仔鼠海马CA2区、CA3区出现大量变性细胞，且细胞排列松散，细胞层数减少。Timm’s染色，正常组仔鼠生后21　d开始出现MF发芽，其吸光度(A)值为0.135　0±0.024　3；铅暴露组仔鼠生后21　d MF发芽的A值为0.096 7±0.024 2，较正常组显著降低(P<0.01)。铅暴露组生后7 d仔鼠海马ZnT-1表达水平显著低于对照组（P<0.01），生后14和21 d与对照组比较差异无统计学意义(P>0.05)。结论：孕期至幼年期低水平铅暴露可能导致仔鼠海马神经元Zn2+失衡和海马神经元胞内Zn2+水平降低，后者可能参与铅暴露导致发育期神经损伤。

关键词: 铅, 锌, 仔鼠, 锌转运体

Abstract: Abstract:Objective To investigate the hippocamal neuron injury in pup rats caused by lead exposure from pregnancy to juvenile stage and the mechanism related to zinc balance. Methods Twenty-five female Wistar rats were randomly divided into control group (n=10) and lead exposure group (n=15).The pup rats of each group were subdivided into three subgroups depending on different observation periods from pregnancy to postnatal 7,14,and 21 d (P7,P14 and P21 subgroups),respectively; each subgroup was composed of 8 pup rats.The female rats in lead exposure group were bred with 0.2% lead acetate in drinking water from pregnancy to P7,P14,and P21 of the pup rats,respectively,while the mothers in control group drank water consistently.The blood and brain lead contents were detected on P7,P14,and P21 of the pup rats in each subgroup.Timm’s staining was used to observe the mossy fiber (MF) in hippocamspus,and immunohistochemistry assay was performed to detect the expression levels of zinc transporter 1 (ZnT-1).Results The blood and brain lead contents in lead exposure subgroups were significantly higher than those in corresponding control subgroups(P<0.01).There were mass of degenerated cells in CA2 and CA3 regions of hippocampus in pup rats after lead exposure,and the nerve cell arrangement was unconsolidated,and the number of cell layers was decreased.The Timm’s staining results showed that MF sprouting presented on P21 of the pups in control group,and the absorbtion(A) value was 0.135 0±0.024 3.The A valueof P21 in lead exposure group was 0.096 7±0.024 2,which was significantly lower than that in control group (P<0.01).The expression of ZnT-1 was significantly decreased on P7 of the pups in lead exposure group compared with control group (P<0.01).There was no significant difference of ZnT-1 expression on P14 and P21 between lead exposure and control group.Conclusion There may exist neuronal zinc imbalance in pup rats after low-level lead exposure from pregnancy to juvenile stage,and it may cause the decrease of the intracellular Zn2+level in hippocampal neurons after lead exposure.

Key words: , lead, zinc, pup rat, zinc transporter

中图分类号:

李海波，刘行，孙美花,梁建民. 孕期至幼年期低水平铅暴露仔鼠海马锌离子平衡的变化及其发育期海马神经元的损伤[J]. 吉林大学学报(医学版), 2013, 39(3): 521-528.

LI Hai-bo,LIU Xing,SUN Mei-hua,LIANG Jian-min. Change of zinc balance in hippocampus of pup rats after lead exposure from pregnancy to juvenile stage and hippocampal neuron injury in development stage[J]. Journal of Jilin University Medicine Edition, 2013, 39(3): 521-528.

[1]	陈良妹, 董晓明, 杨帆, 罗萍. 锌缺乏症对慢性肾脏病发展的影响及其作用机制的研究进展[J]. 吉林大学学报(医学版), 2018, 44(01): 195-199.
[2]	冯安琪, 刘楠, 朴美花, 苑野, 裴爱月, 刘娅, 冯春生. 异氟醚对缺锌APP/PS1转基因阿尔茨海默病模型小鼠海马神经元凋亡的影响[J]. 吉林大学学报(医学版), 2018, 44(01): 24-29.
[3]	郗艳丽, 王洪艳, 白雪松, 杜鹃, 曲丽, 吴启坤, 马洪波, 牛凤兰. 五味子乙素对染铅小鼠驱铅作用及其对驱铅过程中钙、铁和锌水平的影响[J]. 吉林大学学报(医学版), 2015, 41(05): 932-936.
[4]	杨思嘉，陈阳，徐珊珊，潘阳，姚宇航，杨雪玲，李波. 675名中小学生发样中Ca、Fe、Zn和Cu含量的检测及其意义[J]. 吉林大学学报(医学版), 2013, 39(2): 391-395.
[5]	楚洪波，李冬梅，石搏，黄可欣，辛暨丽. 大豆皂苷对糖尿病大鼠心肌组织中NOX4和p22phox表达的影响及其对心肌的保护作用[J]. 吉林大学学报(医学版), 2013, 39(2): 251-254.
[6]	仲楼\|史加海\|曹飞\|陈新民 . ZEB1的表达与肺鳞状细胞癌侵袭及转移的关系[J]. J4, 2012, 38(4): 761-765.
[7]	郭丽, 尹飞, 卢日峰, 孟春阳, 陈强, 黄金长, 杨小玉, 范洪学. 锌对大鼠脊髓缺血再灌注损伤的保护作用[J]. J4, 2010, 36(6): 1003-1006.
[8]	卢应, 吴文杰, 刘娅, 李鸿雁, 张桂英, 王雪. 外源硫酸锌对糖尿病大鼠肝脏损伤的保护作用[J]. J4, 2010, 36(5): 871-874.
[9]	何明蕊, 董宇, 付彤, 孙珉丹. 锌制剂对糖尿病大鼠肾脏和肾脏组织中VEGF、MT及MMP-9表达的影响[J]. J4, 2010, 36(4): 656-659.
[10]	韩长城, 于庆海, 康维钧, 宋波, 唐龙妹. 驱铅益智口服液对铅染毒大鼠的治疗作用[J]. J4, 2009, 35(6): 1061-1064.
[11]	刘微, 王艳春, 范红艳, 沈楠, 任旷. 人参皂苷Rb1对染铅小鼠骨铅含量及行为记忆的影响[J]. J4, 2009, 35(5): 848-851.
[12]	刘忠民，邴国强，李春玲，李萍，华景梅. 运动环境对低浓度铅暴露小鼠记忆能力的影响[J]. J4, 2008, 34(1): 127-130.
[13]	胡秀丽,李才,苗春生，孙波，史经宇. MT蛋奶粉对糖尿病大鼠脂代谢及血清锌和铜水平的影响[J]. J4, 2007, 33(5): 853-855.
[14]	杨昆，迟宝荣，吝春红 . 醋酸铅对小鼠骨髓间充质干细胞生长的影响[J]. J4, 2007, 33(1): 116-118.
[15]	刘瑾，张灵，李晓萌，许莉，汲坤,赵雪俭，李扬. 锌对前列腺癌细胞PC-3M凋亡的影响[J]. J4, 2006, 32(5): 808-811.

孕期至幼年期低水平铅暴露仔鼠海马锌离子平衡的变化及其发育期海马神经元的损伤

Change of zinc balance in hippocampus of pup rats after lead exposure from pregnancy to juvenile stage and hippocampal neuron injury in development stage

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0