关键词: 清道夫受体A, 基因表达, 模型, 动物

Abstract: Objective To study the relationships between nephritic gene expression of scavenger receptor A, lipid metabolism and pathology in adriamycin nephrosis rats.Methods Injecting of adriamycin in veins of the rat′s tail was used to make adriamycin nephrosis model, PCR and RT-PCR were used to measure the gene expression levels of scavenger receptor A in different groups. Results Compared with control group, protein excretory quantity in adriamycin nephrosis group was significantly increased (P<0.05),the levels of serum total protein and albumin were remarkedly decreased (P<0.05), while the levels of TG, TC, HDL, LDL, ApoA, ApoB were increased (P<0.05) . The gene expression value of scavenger receptor A in adriamycin nephrosis group (0.74±0.14)　was higher than that in control group (1.17±0.13) (P<0.01). Glomerulus epithelium foot appeared confluence, endodermis cells proliferated, in some serious ones the basis membrane of glomerulus was unclear, endodermis cell was archoid observed under electron microscope in adriamycin nephrosis rats. Conclusion The gene expression value of scavenger receptor A is lower in normal rats, and the high value of scavenger receptor A is closely related to abnormal of lipid metabolism in adriamycin nephrosis rats. It is also one of the main reasons of pathologic changes of the kidney.

Key words: scavenger receptor A, gene expression, models, animal