吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (01): 7-10.doi: 10.13481/j.1671-587x.20160102

• 基础研究 • 上一篇    下一篇

SUMO及SUMO化修饰蛋白在恶性胶质瘤发生中的作用

齐玲1, 王玮瑶1, 郑中华1, 张以忠1, 赵丽微1, 钟秀宏1, 赵东海1, 杨淑艳1, 杨宁江1, 任旷2, 于洪泉3   

  1. 1. 吉林医药学院病理教研室, 吉林吉林 132013;
    2. 吉林医药学院药理教研室, 吉林吉林 132013;
    3. 吉林大学第一医院神经外科, 吉林长春 130021
  • 收稿日期:2015-07-01 发布日期:2016-01-26
  • 通讯作者: 任旷,教授,硕士研究生导师(Tel:0432-64560301,E-mail:renkuang@sina.com);于洪泉,副主任医师,硕士研究生导师(Tel:0431-81875717,E-mail:hqy1974@163.com) E-mail:renkuang@sina.com;hqy1974@163.com
  • 作者简介:齐玲(1974-),女,吉林省吉林市人,副教授,医学博士,主要从事脑肿瘤方面的研究。
  • 基金资助:

    国家自然科学基金资助课题(81201671);吉林省科技厅国际合作项目资助课题(20140414049GH);吉林医药学院科研启动项目资助课题(201101);吉林省卫生计生委科研计划项目资助课题(2015Z026)

Effects of SUMO and proteins modified by SUMO on occurrence of malignant gliomas

QI Ling1, WANG Weiyao1, ZHENG Zhonghua1, ZHANG Yizhong1, ZHAO Liwei1, ZHONG Xiuhong1, ZHAO Donghai1, YANG Shuyan1, YANG Ningjiang1, REN Kuang2, YU Hongquan3   

  1. 1. Department of Pathology, Jilin Medical University, Jilin 132013, China;
    2. Department of Pharmacology, Jilin Medical University, Jilin 132013, China;
    3. Department of Neurosurgery, First Hospital, Jilin University, Changchun 130021, China
  • Received:2015-07-01 Published:2016-01-26

摘要:

目的: 观察小泛素化相关修饰蛋白质类(SUMO)及SUMO化修饰蛋白在恶性胶质瘤组织中的表达,阐明SUMO化与恶性胶质瘤发生的关系。 方法: 人脑正常组织和恶性胶质瘤组织样品分别作为正常对照组和恶性胶质瘤组,采用蛋白免疫印迹法检测SUMO1、SUMO2、SUMO活化酶(Aos1和Uba2)、SUMO结合酶(UBC9)和SUMO连接酶(PIAS1、PIAS2、PIAS3和Pc2)蛋白的表达水平。 结果: 与正常对照组比较,恶性胶质瘤组织中SUMO1、SUMO2、Aos1、UBC9和PIAS3蛋白表达水平明显升高(P<0.01),Uba2、PIAS1和PIAS2蛋白表达水平明显降低(P<0.01)。 结论: SUMO及SUMO化修饰蛋白可能对恶性胶质瘤的发生起重要的促进作用。

关键词: 泛素化相关修饰蛋白质类, 恶性胶质瘤, 基因表达

Abstract:

Objective: To observe the expressions of small ubiquitin-related modifier protein (SUMO)and related proteins modified by SUMO in the malignant gliomas tissue,and to explore the relationship between sumolation and the occurrence of malignant glioma.Methods: Two samples of normal brain tissues and malignant glioma tissue were prepared as control group and malignant glioma group. The expressions of SUMO1,SUMO2,Aos1,Uba2,UBC9,PIAS1,PIAS2,PIAS3 and Pc2 proteins were detected by Western blotting assay.Results: Compared with control group,the expression levels of SUMO1,SUMO2, Aos1,UBC9 and PIAS3 proteins in malignant glima group were significantly increased (P<0.01), but the expression levels of Uba2,PIAS1 and PIAS2 proteins were decreased(P<0.01). Conclusion: SUMO and poteins modified by SUMO may play an important role in the occurrence of malignant gliomas.

Key words: small ubiquitin-related modifier protein, malignant glioma, gene expression

中图分类号: 

  • R739.41