关键词: 痉挛, 支气管扩张试验, 疾病模型, 动物

Abstract: Objective To explore the linearic regularity of airways′ spasm and spasmolysis in hyperreactive guinea pigs. Methods Asthmatic guinea pig models were established with acetylcholine(Ach). Then the animals inhaled salbutamol. At 0, 15, 30 min and 1 h after inhalation of salbutamol, the animals were sacrificed, and the small and large airways were fixed with formaldehyde and embedded in paraffin wax to acquire the consecutive slides. The pathologic changes of both the large and small airway s were observed and the caliber changes of the airways were determined. Results The diameters of small and large airways of the control guinea pig models (0.41±0.10 and 1.78±0.41 mm,respectively) were significantly short than that in normal(0.55±0.20 and 2.41±0.50 mm, respectively)(P<0.05). Ach aerosol aspiration contracted the small and large airways similarly. After given salbutamol, the large airways began to contract at 15 min (1.99±0.55 mm) and lasted till 1 h (2.25±0.80 mm)（P<0.05）. However, no small airways dilated at 15 min or 30 min and began to dilate until 1 h(0.50±0.14 mm)（P<0.05）. Conclusion In asthmatic guinea pigs, the changes of spasmolysant in large airways and small airways are different, the large airways dilate first and small airways dilate later.

Key words: spasm, bronchodilation test, disease models, animal