关键词: SKOV3细胞, 细胞周期, 细胞凋亡

Abstract: To explore the inhibitory effects of fraction Ⅰ from scorpion venom (SV-Ⅰ) on the growth of SKOV3 ovarian carcinoma cells. MethodsSKOV3 ovarian carcinoma cells were divided into two groups: control group and treatment group with SV-Ⅰ（final concentration as 200，400 and 800 mg•L^-1,respectively）. MTT method was used to detect the inhibitory rate of tumor cells treated with SV-Ⅰ in vitro and colony forming assay was applied to measure the proliferation of tumor cells. Moreover, flow cytometry was further adopted to observe the changes of cell cycle and apoptosis of SKOV3 cells. Results SV-Ⅰ could inhibit the growth of SKOV3 ovarian carcinoma cells significantly at the concentrations of 200, 400 and 800 mg•L^-1 and t he inhibitory rates were 29.87 %，48.11% and 67.77 %， respectively (P<0.01). Furthermore, SV-Ⅰ could inhibit colony forming capacity of SKOV3 ovarian carcinoma cells at 400 and 800 mg•L^-1 and the numbers of colony were 57.00±6.16 and 48.00±4.11, respectively (P<0.01). Compared with control, cells in S stage were decreased and cells in G₂+M stage were increased obviously (P<0.01) after SV-Ⅰ was given at 400 mg•L^-1, while cells in G₂+M stage were decreased (P<0.01) and cells in G₁ stage had a ascending tread after treated with 800 mg•L^-1 SV-Ⅰ. Furthermore, apoptosis could be induced obviously by SV-Ⅰ at 400 and 800 mg•L^-1 compared with control(P<0.01). Conclusion SV-Ⅰ could inhibit the proliferation and growth of SKOV3 ovarian carcinoma cells and the changes of cell cycle and apoptosis may be important mechanism of inhibitory effects of SV-Ⅰ.

Key words: SKOV3 ovarian carcinoma, Cell cycle, Apoptosis