关键词: 高级, 结缔组织生长因子, 糖尿病, 实验性, 纤维化, 氨基胍, 葛根素

Abstract: To observe the effect of advanced glycosylation end products (AGEs) on gene expression of connective tissue growth factor (CTGF) in NIH Swiss mouse embryo fibroblasts(NIH/3T3),and to assess the intervention actions of aminoguanidine(AG) and puerarin(Pue) on CTGF mRNA expression in NIH/3T3.Methods AGEs were synthesized by coincubation of BSA with glucose.The AGEs content was measured by fluorescence spectroscopy.NIH/3T3 cells were treated with AGEs (prepared with 20，50，80 mmol•L^-1 glucose) for 24 h.The NIH/3T3 cells were treated with AGEs (prepared with 50 mmol•L^-1 glucose) for 0，6，12，24 and 48 h.The intervention actions of AG and Pue with different concentration (0.25，0.5，1.0 and 1.5 g•L^-1) were evaluated.The CTGF mRNA expression in NIH/3T3 was determined by RT-PCR. Results Compared with BSA control,the CTGF mRNA expression levels in NIH/3T3 were increased by treatment with AGEs (prepared with 20, 50, 80 mmol•L^-1 glucose) for 24 h (P<0.01),among them 50 mmol•L^-1 glucose was highest.Compared with 0 h,the CTGF mRNA expression levels in NIH/3T3 were increased by treatment with AGEs (prepared with 50 mmol•L^-1 glucose) for 6,12,24,48 h (P<0.01),among them 24 h was highest.Compared with AGEs (prepared with 50 mmol•L^-1 glucose) for 24 h,AG and Pue with different concentrations significantly decreased the CTGF mRNA expression in NIH/3T3 (P<0.01).Conclusion AGEs up-regulates CTGF mRNA expression in NIH/3T3,which is related with glucose concentration and acting time.Based on the above mentioned,AGEs may promote the fibrotic process of diabetic complications.AG and Pue can attenuate the profibrotic effects of AGEs.

Key words: advanced, connective tissue growth factor, diabetes mellitus, experimental, fibrosis, aminoguanidine, puerarin