关键词: HSP-MUC1, CpG ODN, 干扰素 , 疫苗, 合成, 佐剂, 免疫

Abstract: To screen one kind of antitumor immunoadjuvant that could enhance the anti-tumor activity of heat shock fusion protein(HSP-MUC1).Methods MUC1-positive tumor bearing mice were subcutaneously injected by HSP-MUC1 combined with a novel CpG ODN or hIFNα2b,four groups were set up respectively as PBS,HSP-MUC1,CpG ODN(IFNα2b) and HSP-MUC1+CpG ODN(HSP-MUC1+IFNα2b).The tumor incidence and tumor volume in various groups were observed and calculated. Results The tumor incidence and tumor weight of mice in HSP-MUC1+CpG ODN group were lower than those in PBS group and HSP-MUC1 group(P<0.05); CpG ODN alone also displayed the similar anti-tumor effect as HSP-MUC1+CpG ODN,it could decrease the tumor incidence and tumor weight of mice compared with PBS and HSP-MUC1(P<0.05); And the tumor incidence and tumor weight of mice in HSP-MUC1+hIFNα2b group had no difference with those in PBS group and HSP-MUC1 group(P> 0.05). Conclusion CpG ODN could enhance the anti-tumor activity of HSP-MUC1 but need to be further determined for its optimal injection time；and human IFNα2b could not enhance the anti-tumor activity of HSP-MUC1.

Key words: HSP-MUC1, CpG ODN, interferon-alpha, vaccine, synthetic, adjuvant, immunologic