关键词: 人前列腺癌细胞, 多药耐药相关蛋白质类, P-糖蛋白, 抗肿瘤药

Abstract: To establish a multidrug resistance cell line from human prostatic cancinoma PC3M cell line.Methods The human prostatic cancinoma cell line PC3M was exposed to cisplatin with high dose and the concentration of cisplatin was increased gradually and then multidrug resistance cell line (PC3M/ CDDP) was established.The relative resistance was tested with MTT assay.The expressions of P-glycoprotein (P-gP) and multidrug resistance-related protein(MRP) were observed with immunocytochemical method.Results The IC₅₀ of cisplatin,mitomycin,doxorubicin,5-fluorouracil,vincristine on PC3M/CDDP cell were (0.603±0.102),(0.201±0.056),(0.267±0.067),(1.676±0.321) and (0.657±0.227) mg•L^-1,respectively,there was significant difference of IC₅₀ between PC3M/CDDP cell and PC3M cell(P<0.05).The MRP and P-gP expressions of PC3M/CDDP cell were higher than those of PC3M cells(P<0.05).The proliferation rate of PC3M/CDDP cell (40.5h) had no obvious difference when compared with that of PC3M cell (48.6 h) (P>0.05). Conclusion A multidrug resistance cell line (PC3M/CDDP) is established from human prostatic cancinoma PC3M cell line.The PC3M/CDDP cell was resistant to mulidrug,and no change on cell proliferation.It can be used to downstream experiment.

Key words: human prostatic cancinoma cells, multidrug resistance-associated proteins, P-glycoprotein, antineoplastic agents