关键词: 胶质瘤细胞, 凋亡相关基因, 氧化应激

Abstract: To observe the effects of Okinawa Habu apoxin protein-1 (OHAP-1) on the proliferation inhibition of rat C6 glioma cells and its mechanisms. Methods MTT colorimetric analysis was used to measure the inhibitory effect of OHAP-1 with different doses（2.5,5.0,and 10.0 mg•L-1） on C6 glioma cells .RT-PCR was used to evaluate the mRNA expressions of bcl-2 and bax genes.The activity of superoxide dismutase (SOD) and the level of maleicdialdehyde (MDA) in the C6 glioma cells were also examined. Results The proliferation of C6 glioma cells was significantly inhibited by different doses of OHAP-1（2.5,5.0,and 10.0 mg•L^-1）.The inhibitory rate were 49.77%,67.65％,and 76.42%，respectively. The inhibitory rate in 2.5,5.0, and 10.0 mg•L^-1 groups were higher than that in control group(P<0.001).With the increasing of the dose of OHAP-1,bax mRNA expression increased,and bcl-2 mRNA decreased markedly,the ratio of bcl-2/bax mRNA also decreased.In 2.5,5.0, and 10.0 mg•L^-1 OHAP-1 groups,MDA concentrations increased significantly compared with control group(P<0.01),and SOD decreased (P<0.01); In 5.0 and 10.0 mg•L^-1 OHAP-1 groups,MDA concentrations were higher than those in 2.5 mg•L^-1 OHAP-1 and control groups (P<0.001);in 10.0 mg•L-1 OHAP-1 group, SOD activity was lower than those in 2.5 mg•L^-1 and control groups (P<0.001); in 10.0 mg•L^-1 OHAP-1 group, SOD activity was lower than those in 5.0 ,2.5 mg•L^-1OHAP-1 and control groups (P<0.001). Conclusion The proliferation inhibition of C6 glioma cell induced by OHAP-1 may be related to the expression of bcl-2/bax mRNA decreasing resulted from oxidative stress.

Key words: glioma, apoptosis associated gene, oxidative stress