阿司匹林和塞来昔布对乳腺癌细胞MCF-7增殖的影响

doi:吴阶平医学基金资助课题（320，2730，0501

摘要/Abstract

摘要： 目的：观察阿司匹林和塞来昔布及二者分别联合阿那曲唑对乳腺癌细胞MCF-7增殖的影响，探讨并比较阿司匹林和塞来昔布的抗肿瘤作用。方法：MTT法检测不同浓度的阿司匹林（2.5 、5.0和10.0 mmol•L^-1）和塞来昔布（30、60和120 μmol•L^-1）分别作用人乳腺癌细胞株MCF-7不同时间（24、48和72 h）的细胞增殖抑制率，以不含药物的培养液作为阴性对照组，以阿霉素作为阳性对照组。MTT法检测不同浓度阿那曲唑（0.5和1.0 mmol•L^-1及其分别联合不同浓度阿司匹林（2.5、5.0和10.0 mmol•L^-1）和塞来昔布（30、60和120 μmol•L^-1）作用于MCF-7细胞48 h的细胞增殖抑制率，以不含药物组为阴性对照组。结果：①阿司匹林各剂量组细胞增殖抑制率随药物浓度的增加、作用时间的延长而提高(P<0.01)。②塞来昔布各剂量组的细胞增殖抑制率随药物浓度的增加、作用时间的延长而升高(P<0.01)。③10 mmol•L^-1阿司匹林和120 μmol•L^-1塞来昔布单独作用72 h抑制率分别达68.88%和87.00％；阳性对照组阿霉素2.0 μmol•L^-1作用72h抑制率达86.30％。④0.5 μmol•L^-1阿那曲唑与阿司匹林联合各剂量组的抑制率均明显高于0.5 μmol•L^-1阿那曲唑单药组(P<0.05)，0.5 μmol•L^-1阿那曲唑联合10.0 mmol•L^-1阿司匹林对MCF-7的抑制率明显高于0.5 μmol•L^-1阿那曲唑联合2.5、5.0 mmol•L^-1阿司匹林(P<0.05)。0.5 μ mol•L^-1阿那曲唑分别与60、120 μmol•L^-1塞来昔布联合组的抑制率明显高于0.5 μmol•L^-1阿那曲唑单药组及0.5 μmol•L^-1阿那曲唑与30 μmol•L^-1塞来昔布联合组的抑制率(P<0.05)，0.5 μmol•L^-1阿那曲唑联合120 μmol•L^-1塞来昔布对MCF-7的抑制率明显高于0.5 μmol•L^-1阿那曲唑联合60 μmol•L^-1塞来昔布(P<0.05)。1.0 μmol•L^-1阿那曲唑分别联合10.0mol•L^-1阿司匹林、60和120 μmol•L^-1塞来昔布各组的抑制率明显高于1.0 μmol•L^-1阿那曲唑单药组(P<0.05)，1.0 μmol•L^-1阿那曲

关键词: 阿司匹林, 塞来昔布, 阿那曲唑, 乳腺肿瘤, 人乳腺癌细胞, MCF-7

Abstract: Objective To discuss and compare the anti-tumor effects of aspirin and celecoxib on breast cancer cell MCF-7 through investigating the effects of aspirin ，celecoxib, and combined with anastrozole respectively on the growth of human breast cancer cell MCF-7.Methods The human breast cancer cell MCF-7 were treated with 2.5 ,5.0 ,and 10.0 mmol•L^-1　aspirin and 30 ,60 ,and 120 μmol•L^-1 celecoxib for 24，48，and 72 h respectively, the MCF-7 without treatment with drug was used as negative control group, the MCF-7 treated by ADM was used as positive control group, the inhibitory effect was detected by MTT assay. Besides, the MCF-7 cells were treated by anastrozole(0.5 and 1.0 μmol•L^-1), anastrozole(0.5 and 1.0 μmol•L^-1) combined with aspirin(2.5 ,5.0 ,and 10.0 mmol•L^-1)or celecoxib(30，60，and 120 μmol•L^-1) for 48 h, respectively, the inhibitory rate was detected by MTT assay. Results ① The inhibitory rate of the MCF-7 cell line treated with aspirin was reduced compared with controls, which was in time-dependent and dose-dependent manner(P<0.01).②The inhibitory rate of the MCF-7 cell line treated with celecoxib was reduced compared with controls, which was in time-dependent and dose-dependent manner(P<0.01).③The inhibitory rates of 10 mmol•L^-1 aspirin and 120 μmol•L^-1 celecoxib on MCF-7 were 68.88% and 87.00% when treated for 72 h, the inhibitory rate of 2.0 μmol•L^-1 ADM on MCF-7 was 86.30% when treated for 72 h.④The inhibitory rate of 0.5 μmol•L^-1 anastrozole combined with aspirin on MCF-7 was increased compared with that of anastrozole single (P<0.05).The inhibitory rate of 0.5 μmol•L^-1 anastrozole combined with 10.0 mmol•L^-1 aspirin on MCF-7 was increased compared with that of 0.5 μmol•L^-1 anastrozole combined with 2.5 mmol•L^-1 or 5.0 mmol•L^-1 aspirin(P<0.05).The inhibitory rates of 0.5 μmol•L^-1 anastrozole combined with 60 μmol•L^-1 or 120 μmol•L^-1 celecoxib on MCF-7 were increased compared with that of 0.5 μmol•L^-1 anastrozole single and 0.5 μmol•L^-1 anastrozole combined with 30 μmol•L^-1 celecoxib (P<0.05).The inhibitory rate of 0.5 μmol•L^-1 anastrozle combined with 120 μmol•L^-1 celecoxib on MCF-7 was increased compared with that of 0.5 μmol•L^-1 anastrozole combined with 60 μmol•L^-1 celecoxib .The inhibitory rates of 1.0 μmol•L^-1 anastrozole combined with 10.0 mmol•L^-1 aspirin, 60 μmol•L^-1 or 120 μmol•L^-1 celecoxib on MCF-7 were increased compared with that of 1.0 μmol•L^-1 anastrozole single(P<0.05).The inhibitory rate of 1.0 μmol•L^-1 anastrozole combined with 120 μmol•L^-1 celecoxib on MCF-7 was increased compared with that of 1.0 μmol•L^-1 anastrozole combined with 60 μmol•L^-1 celecoxib(P<0.05).Conclusion Aspirin and celecoxib have inhibitory effects on the growth of human breast cancer cell MCF-7 in a dose- and time-dependent manner. There is a synergistic effect on MCF-7 when anastrozole is combined with aspirin or celecoxib .

Key words: aspirin, celecoxib, anastrozole, breast neoplasms, human breast cancer cell , MCF-7

中图分类号:

R737.9

张岩，王杨，闫秀欣. 阿司匹林和塞来昔布对乳腺癌细胞MCF-7增殖的影响[J]. J4, 2007, 33(5): 901-904.

ZHANG Yan, WANG Yang, YAN Xiu-xin. Effect of aspirin and celecoxib on proliferation of breast cancer cell line MCF-7[J]. J4, 2007, 33(5): 901-904.

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阿司匹林和塞来昔布对乳腺癌细胞MCF-7增殖的影响

Effect of aspirin and celecoxib on proliferation of breast cancer cell line MCF-7

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