关键词: 肝细胞生长因子, 格尔德霉素, 增殖 , 基因表达

Abstract: Objective To investigate the effects of geldanamycin (GDM) on the proliferation and relative gene expression in glioma cells induced by hepatocyte growth factor (HGF).Methods U251 MG and U87 MG of human malignant glioma cells were cultivated by cell culture technique.After cultivated in the presence of HGF for 24 h,U251 MG and U87 MG cells were cultivated for another 48 h in the presence of GDM at different concentrations (50,250,500, and 1 000 nmol•L^-1).The inhibitory rates of growth were examined by MTT essays.The experiment was divided into normal cells,cells+HGF (30 μg•L^-1),cells+GDM (50,250,500 and 1 000 nmol•L^-1　 and cells+HGF+GDM and paclitaxel groups.The relative gene mRNA expression level was detected with RT-PCR methods.Results After U251 MG and U87 MG cells were treated with HGF for 24 h,the proliferative rates were 0.139±0.07 and 0.242±0.167, respectively;and 50,250,500 and 1 000 nmol•L^-1 GDM had inhibitory effects on U251 and U87 cells,the inhibitory rates were 0.029±0.028,0.027±0.017,0.312 ±0.084,0.339±0.047 and 0.116±0.069,0.222±0.191,0.269±0.056,0.276±0.031; the inhibitory rates of paclitaxel on U251 MG and U87 MG cells were 0.075±0.062 and 0.071±0.044.The results of RT-PCR appered that the HGF and c-Met mRNA in U251 MG and U87 MG cells in HGF group were lower than those in normal group(（P<0.05）,but the HGF and c-met mRNA in U251 MG and U87 MG cells in HGF+GDM group were lower than those in HGF group (P<0.05).Conclusion GDM could inhibit the proliferation of glioma cells induced by HGF and regulate the relative gene expression.

Key words: hepatocyte growth factor, geldanamycin, proliferation , gene expression