关键词: 细胞凋亡, 机制

Abstract: Abstract:Objective To study the effect of small heterocyclic compound 8-oxo-3-thiomorpholino-8H-acenaphtho ［1,2-b］ pyrrole-9-carbonitrile (S1) on apoptosis of human androgen independent prostatic carcinoma cells line(PC3) and its mechanism.Methods PC3 cells were cultivated, and divided into different groups: 10.00,5.00,1.00,0.50,0.10,0.05 and 0.01 μmol•L^-1 S1 groups, meanwhile, PC3 control group and cyclophosphamide group were set up. MTT was used to detect the inhibitory rate of PC3 cell proliferation.Flow cytometry was used to detect the inducing effect of S1 on apoptosis of PC3 cells.Caspase 3, 8, 9 kits were used to detect apoptosis route.Results The inhibitory rates of PC3 cells induced by 0.10-10.00 μmol•L^-1 S1 were significantly higher than that in cyclophosphamide group （P<0.01）.The apoptotic rates induced by 5.00 and 10.00 μmol•L^-1S1 were significantly higher than that in PC3 control group（P<0.01）.When PC3 cells were treated with 10.00 μmol•L^-1 S1 for 6 h, the activity of Caspase3 was significantly higher than that in PC3 control group（P<0.01）.When PC3 cells were treated with 10.00 μmol•L^-1 S1 for 4-6 h, the activities of Caspase 9 were significantly higher than that in control group（P<0.01）, but the activity of Caspase 8 did not change significantly.Conclusion S1 compound can induce apoptosis of PC3 cell line and death,and its mechanism may be related to mitochondria.

Key words: apoptosis, mechanism