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小分子化合物S1诱导PC3细胞凋亡的作用

吴丛梅1, 杨 清2, 金顺子3   

  1. 1.长春工业大学生物工程学院, 吉林 长春 130012;2.吉林大学第一医院检验科,吉林 长春 130021; 3.吉林大学公共卫生学院 卫生部放射生物学重点实验室, 吉林 长春 130021
  • 收稿日期:2007-10-26 修回日期:1900-01-01 出版日期:2008-05-28 发布日期:2008-05-28
  • 通讯作者: 金顺子

Effect of small heterocyclic compound S1 on inducing PC3 cell line apoptosis

WU Cong-mei1, YANG Qing2, JIN Shun-zi3   

  1. 1.School of Bioengineering, Changchun University of Technology, Changchun 130012, China; 2.Department of Laboratory, First Hospital, Jilin University, Changchun 130021, China; 3.MH Radiobiology Research Unit,School of Public Health,Jilin University,Changchun 130021,China
  • Received:2007-10-26 Revised:1900-01-01 Online:2008-05-28 Published:2008-05-28
  • Contact: JIN Shun-zi

摘要: 目的:研究小分子化合物8-氧-8H-苊并[1,2-b]吡咯-9-腈的氨基或卤素取代衍生物(S1)对人雄性激素非依赖型前列腺癌PC3细胞的作用及机制。方法:常规培养PC3细胞,分为10.00、5.00、1.00、0.50、0.10、0.05和0.01 μmol•L-1 S1化合物组,同时设培养肿瘤细胞对照组和抗癌药物环磷酰胺(5.00 μmol•L-1)对照组,采用MTT法检测PC3细胞增殖抑制率,流式细胞术检测诱导PC3细胞凋亡作用,Caspase 3、8和9试剂盒检测细胞凋亡途径。结果:0.10~10.00 μmol•L-1 S1化合物组,PC3细胞生长抑制率明显高于环磷酰胺对照组(P<0.01);5.00和10.00 μmol•L-1S1化合物组PC3细胞凋亡率明显高于培养肿瘤细胞对照组(P<0.01);10.00 μmol•L-1S1化合物作用于PC3细胞后不同时间(1、2、4及6 h)细胞Caspase 3和Caspase 9活性均显著高于培养肿瘤细胞对照组(P<0.01),Caspase 8活性未见明显改变。结论:S1化合物能够通过诱导PC3细胞凋亡而导致细胞死亡,其作用机制是线粒体途径,进一步证实S1化合物作为肿瘤治疗药物的可行性。

关键词: 细胞凋亡, 机制

Abstract: Abstract:Objective To study the effect of small heterocyclic compound 8-oxo-3-thiomorpholino-8H-acenaphtho [1,2-b] pyrrole-9-carbonitrile (S1) on apoptosis of human androgen independent prostatic carcinoma cells line(PC3) and its mechanism.Methods PC3 cells were cultivated, and divided into different groups: 10.00,5.00,1.00,0.50,0.10,0.05 and 0.01 μmol•L-1 S1 groups, meanwhile, PC3 control group and cyclophosphamide group were set up. MTT was used to detect the inhibitory rate of PC3 cell proliferation.Flow cytometry was used to detect the inducing effect of S1 on apoptosis of PC3 cells.Caspase 3, 8, 9 kits were used to detect apoptosis route.Results The inhibitory rates of PC3 cells induced by 0.10-10.00 μmol•L-1 S1 were significantly higher than that in cyclophosphamide group (P<0.01).The apoptotic rates induced by 5.00 and 10.00 μmol•L-1S1 were significantly higher than that in PC3 control group(P<0.01).When PC3 cells were treated with 10.00 μmol•L-1 S1 for 6 h, the activity of Caspase3 was significantly higher than that in PC3 control group(P<0.01).When PC3 cells were treated with 10.00 μmol•L-1 S1 for 4-6 h, the activities of Caspase 9 were significantly higher than that in control group(P<0.01), but the activity of Caspase 8 did not change significantly.Conclusion S1 compound can induce apoptosis of PC3 cell line and death,and its mechanism may be related to mitochondria.

Key words: apoptosis, mechanism

中图分类号: 

  • Q503