新型免疫调节性寡聚脱氧核苷酸的序列设计和筛选分析

doi:国家自然科学基金资助课题（30771977）

新型免疫调节性寡聚脱氧核苷酸的序列设计和筛选分析

张雪松¹，胡大利²，孙然²，杨光²，王华颖³，王丽颖³，于永利²

1.吉林省血液中心成分制备科，吉林长春 130033；2.吉林大学基础医学院免疫学教研室，吉林长春130021；3.吉林大学基础医学院分子生物学教研室，吉林长春 130021

收稿日期:2007-09-21 修回日期:1900-01-01 出版日期:2008-05-28 发布日期:2008-05-28
通讯作者: 王丽颖；于永利

Sequence designation and screening of novel immunoregulatory oligodeoxynucleotides

ZHANG Xue-song¹, HU Da-li², SUN Ran², YANG Guang²,WANG Hua-ying³, WANG Li-ying³, YU Yong-li²

1.Division of Constituent Preparation, Blood Center of Jilin Province, Changchun 130033, China; 2.Department of Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China; 3.Department of Molecular Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China

Received:2007-09-21 Revised:1900-01-01 Online:2008-05-28 Published:2008-05-28
Contact: WANG Li-ying, YU Yong-li

摘要/Abstract

摘要： 目的：探讨免疫调节性寡聚脱氧核苷酸（ODN）设计原则和筛选方法，为研究和开发新型免疫调节性ODN提供参考。方法：利用含有CpG基序的免疫刺激性寡聚脱氧核苷酸（CpG ODN）作为刺激物，将人外周血单个核细胞（hPBMC）作为研究对象，以³H-TdR掺入法检测CpG ODN诱导的细胞增殖水平，对自行设计的ODN的免疫调节活性进行筛选分析。结果：通过对两批免疫调节性ODN的设计和筛选，得到具有较强免疫抑制活性的ODN 667B，与CpG BW006组相比，其抑制率达到66.3%（P<0.01），并具有浓度相关性。结论：总结出免疫调节性ODN的设计原则，同时证明筛选方法可以用于免疫调节性ODN的筛选，为进一步研究开发新型免疫调节性ODN奠定了基础。

关键词: 寡聚脱氧核苷酸, CpG ODN, 人外周血单个核细胞

Abstract: Abstract:Objective To study the designing principles and screening method of immunoregulatory oligodeoxynucleotides (ODN) and provide information for the research and development of novel immunoregulatory ODN.Methods The human peripheral blood monouclear cells (hPBMC) were stimulated by CpG ODN and/or immunoregulatory ODN.The CpG ODN-induced cell proliferation was examined by thymidine incorporation to evaluate the immunoregulatory activity of self-designed ODNs.Results ODN 667B with relatively high immunosuppressive activity was obtained by designing and screening of two groups of immunoregulatory ODNs.Compared with CpG BW006 group, the inhibitory rate of 667B reached 66.3% (P<0.01) and the suppression was concentration-dependent. Conclusion The designing principle of immunoregulatory ODN is summaried and the screening method is proved to be available.The results set the foundation for the research and development of novel immunoregulatory ODN.

Key words: oligodeoxynucleotide, CpG ODN, human PBMC

中图分类号:

R392.11

张雪松，胡大利，孙然，杨光，王华颖，王丽颖，于永利. 新型免疫调节性寡聚脱氧核苷酸的序列设计和筛选分析[J]. J4, 2008, 34(3): 389-392.

ZHANG Xue-song, HU Da-li, SUN Ran, YANG Guang,WANG Hua-ying, WANG Li-ying, YU Yong-li. Sequence designation and screening of novel immunoregulatory oligodeoxynucleotides[J]. J4, 2008, 34(3): 389-392.

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