关键词: 15-脱氧前列腺素, 人肺癌PLA-801D细胞株, 细胞凋亡, 细胞周期, 顺铂

Abstract: Abstract:Objective To investigate the effects of 15-deoxy-Δ12,14-prostaglandin J₂(15d-PGJ₂) and DDP on the growth of human pulmonary carcinoma PLA-801D cells and the mechanisms of apoptosis.Methods The human pulmonary carcinoma PLA-801D cells were selected and added to each well of 96-well place and cultivated for 24 h.Then the cells were treated with different concentrations of 15d-PGJ₂(0,5,10,20,40 and 80 μg•L^-1) or 15d-PGJ₂ combined with DDP(3 mg•L) for 24 h.0 μg•L^-1 15d-PGJ₂ group was control group.The morphological changes of cells were observed under inverted microscope.Microculture tetrazolium (MTT)dye was applied to detect the proliferation of the human pulmonary carcinoma PLA-801D cells treated with 15d-PGJ₂ and DDP.Diphenylamine assay (DPA) was used to evaluate the activation.Flow cytometry assay( FCM) was used to detect the apoptosis proportion and the changes of cell cycle.Results When the human pulmonary carcinoma PLA-801D cells were treated with low- concentration 15d-PGJ₂ alone( 5,10 and 20 μg•L^-1), no significant difference was observed in the inhibitory rate of cell growth and the apoptotic indexes such as the apoptosis proportion, the percent of DNA fragmentation and the activity of caspase-3 compared with control roup（P<0.05）；when 15d-PGJ₂ concentration was 40 μg•L^-1 ,the inhibitory rate of cell growth the apoptosis proportion and the percent of DNA fragmentation were higher than those in control group （P<0.01）.While the cells were treated with 15d-PGJ₂ (10 μg•L^-1)combinated with DDP(3 mg•L^-1), the inhibitory rate of cell growth, the apoptosis proportion, the percent of DNA fragmentation and the activity of caspase-3 were significantly higher compared with 15d-PGJ₂ alone（P<0.01）.when the human pulmonary carcinoma PLA-801D cells were treaed with 15d-PGJ₂ alone for 24 h, and the 15d-PGJ₂ concentration was 40 μg•L^-1, the cell proportion in G₀/G₁ phase was significantly higher than that in control group,and the cell proportion in S and G₂/M phase was greatly lower than that in control group（P<0.01）.Conclusion High- concentration 15d -PGJ₂ can induce the apoptosis of human pulmonary carcinoma PLA-801D cells , but the effects inducing apoptosis of 15d -PGJ₂ alone are weaker than those of 15d -PGJ₂ low- concentration combined with DDP.

Key words: 15d -PGJ_2<, sub>, human pulmonary carcinoma PLA-801D, apoptosis, cell cycle, cisplatin