关键词: 神经保护作用, 万拉法新, 氟西汀, 谷氨酸, 神经元

Abstract: Abstract:Objective To investigate the neuroprotection of antidepressants venlafaxine and fluoxetine on the hippocampus neurons injured by glutamate.Methods Primary cultured hippocampus neurons were injured with glutamate to mimic the pathological changes in depression.Four groups were divided as control, glutamate, venlafaxine and fluoxetine administration group.The survival rate of neurons was detected with MTT method.LDH release and SOD activity were detected by spectrophometry.Results The survival rate of neurons injured with the excitatory neurotoxicity of glutamate decreased to 50% of control (P<0.05).The survival rates of neurons injured with glutamate increased dramatically after administration with venlafaxine as compared with those in glutamate group (P<0.05 or P<0.01).However, the survival rates of neurons were not increased after administration with fluoxetine.The LDH release of neurons injured with glutamate increased significantly, which was 7.4-fold of control group.However, after administration with two kinds of antidepressants LDH release from neurons injured with glutamate decreased in varying degrees as compared with that in glutamate group.The SOD activity of neurons in hippocampus injured with glutamate decreased significantly（P<0.01）, nevertheless, the administration of venlafaxine can reverse the parameter（P<0.001）, but the activities of SOD in fluoxetine groups (100 and 500 μmol•L^-1)decreased significantly (P<0.05).Conclusion Antidepressant venlafaxine and fluoxetine both have certain neuroprotection, however, venlafaxine has greater neuroprotection compared with fluoxetine.

Key words: neuroprotection, venlafaxine, fluoxetine, glutamic acid, neurons