关键词: TRAIL, 细胞凋亡, 细胞周期

Abstract: To investigate the effect of recombinant human TNF-related apoptosis-inducing ligand (rhTRAIL) protein on the growth and apoptosis of meL anoma cell line A-375. Methods The A-375 cells were divided into four groups including control group and rhTRAIL 0.25,0.50,and 1.00 mg•L^-1 groups and the morphological changes of A-375 cells were observed by contrast phase microscope after treated with different doses of rhTRAIL. The survival fraction of A-375 cells was measured by MTT assay; Apoptotic rate was determined by TUNEL method and the cell cycle and apoptosis index were analyzed by technique of flow cytometry (FCM ). Results The cells in control group were fusiform adherence or half adherence and abundant,the state of some cells in rhTRAIL group was worse than that in control group.The inhibitory rates of growth in rhTRAIL groups with different doses (0.25,0.50,1.00 mg•L^-1 ) were (15.36±3.21)%,(27.34±2.53)%,and (43.68±3.51)%,respectively,there were significant differences between three groups (P<0.05).The FCM results showed that the rhTRAIL could induce the apoptosis in dose-dependent manner,and the apoptotic rates were 6.68 %,24.59 %,and 28.91 %,respectively, when the rhTRAIL doses were 0.25,0.50,and 1.00 mg•L^-1,there were significant differences between three groups (P<0.05). The G₁ phase cell propotions in rhTRAIL 0.25,0.50,and 1.00 mg•L^-1　groups（45.26%,60.67%,69.10 %） were higher than that in control group（37.41 %）. Conclusion The　 rhTRAIL has the significant effect on inhibiting the growth of A-375 cells，and the inhibitory rate is dose-dependent.The mechanism may be concerned with cell cycle arrest and inducing apoptosis upon A-375 cells.

Key words: TRAIL, apoptosis, 　cell cycle