关键词: 牙髓细胞, 细胞增殖

Abstract: Objective To investigate the effect of different concenrtrations of basic fibroblast growth factor (bFGF) on proliferation of human dental pulp cell in vitro,and to find out the most effective concentration of bFGF. Methods Dental pulp cells were isolated from dental pulp tissue explants.The pulp cells were divided into 5 groups randomly,bFGF was added into each group until the ultimately concentrations were 0.1,1.0,10.0 and 100.0 μg•L^-1respectively while the group without bFGF as control group. The effects of bFGF on dental pulp cells were assayed by absorbency A and relative growth rate(RGR) with MTT colorimetric method. Results bFGF at concentrations of 1.0-100.0 μg•L^-1 promoted the cell proliferation (P<0.01). The difference between 10.0 μg•L^-1 and 1.0 μg•L^-1 bFGF groups was significant（P<0.01）,while that was not obvious between 10.0 μg•L^-1 and 100.0 μg•L^-1 bFGF groups（P>0.05）. Conclusion bFGF has the capability of promoting the proliferation of human dental pulp cells,and the smallest effective concentration is 1.0 μg•L^-1,the most strong cell proliferation takes place at bFGF concentration of 10.0 μg•L^-1.

Key words: dental pulp cells, cell proliferation