关键词: 原癌基因蛋白质c-met, 增殖细胞核抗原, 免疫组织化学

Abstract: To investigate the expression of hepatocyte growth factor receptor(c-met) in cervical cancer,and analyze its relationship with proliferating cell nuclear antigen (PCNA). Methods Immunohistochemistry (S-P method) was adopted to detect c-met and PCNA protein expressions in 14 cases of chronic cervicitis and 50 cases of cervical cancer. Results In chronic cervicitis,the positive rate of c-met was 14.3%(2/14),but in all of cervical cancer was 62.0%(31/50), the difference of c-met expression was significant between chronic cervicitis and cervical cancer (P<0.05).The experession rete of c-met in low differentiation group was higher than that in high differentiation group(P<0.05).The expression of c-met in lymph node metastasis positive group was higher than that in lymph node metastasis negative group(P<0.05);there was no significant difference between squemous cell carcinoma group and adenocarcinoma group（P>0.05）;there was also no significant difference between clinical stage Ⅰ group and clinical stage Ⅱ group.In chronic cervicitis,the PCNA index was 24.79%±5.10%,but in cervical cancer the PCNA index was 39.90%±15.46%,the difference of PCNA index was significant between chronic cervicitis and cervical cancer (P<0.05).The lower of tissure differentiation degree and higher of clinical stage,the higher of PCNA expression(P<0.05).There was no significant difference of the expression of PCNA between squemous cell carcinoma group and adenocarcinoma group(P>0.05).The PCNA index in c-met expression positive tumor was significantly higher than that in the negative ones(P<0.01). Conclusion The expression of c-met has strong relationship with PCNA,it could serve as a prognostic factor of cervical cancer.

Key words: proto-oncogene protein c-met, proliferating cell nuclear antigen, immunohistochemistry