HIF-1α和MVD在卵巢上皮性肿瘤中的表达及意义

doi:吉林省科技厅科技发展计划项目资助课题（20

HIF-1α和MVD在卵巢上皮性肿瘤中的表达及意义

付莉¹，刘爱民¹，冯卫²，王爱绘¹，王冰冰¹，杨丽晓¹，张茜¹

1. 吉林大学第二医院妇产科，吉林长春 130041；2. 吉林大学第一医院骨关节二科，吉林长春 130021

收稿日期:2008-08-18 修回日期:1900-01-01 出版日期:2009-03-28 发布日期:2009-08-14
通讯作者: 刘爱民

Expressions of hypoxia inducible factor - 1α and microvessel density in ovarian epithelial tumor tissues and significance

FU Li¹，LIU Ai-min¹，FENG Wei²，WANG Ai-hui¹，WANG Bing-bing¹，YANG Li-xiao¹，ZHANG Qian¹

1.Department of Obstetrics and Gynecology,Second Hospital,Jilin University,Changchun 130041,China;2.Second Department of Osteoarthrosis,First Hospital,Jilin University,Changchun 130021,China

Received:2008-08-18 Revised:1900-01-01 Online:2009-03-28 Published:2009-08-14
Contact: LIU Ai-min

摘要/Abstract

摘要： 目的：检测卵巢上皮性肿瘤组织中缺氧诱导因子-1α（HIF-1α）和微血管密度（MVD）的表达，探讨HIF-1α和MVD在卵巢上皮性肿瘤发生、发展中的作用。方法：237例卵巢上皮性肿瘤患者，包括浆液性囊腺瘤79例、交界性浆液性囊腺瘤12例、浆液性囊腺癌51例、黏液性囊腺瘤67例、交界性黏液性囊腺瘤9例、黏液性囊腺癌19例。其中浆液性囊腺癌和黏液性囊腺癌按FIGO的手术-病理分期为：Ⅰ期13例，Ⅱ期26例，Ⅲ期19例，Ⅵ期12例。采用免疫组织化学方法检测上述卵巢上皮性肿瘤组织中HIF-1α表达和MVD，并将良性肿瘤、交界性肿瘤和恶性肿瘤，以及恶性肿瘤不同分期之间的HIF-1α表达和MVD值进行对比。同时对HIF-1α的表达和MVD值之间的相关性进行分析。结果：卵巢交界性浆液性囊腺瘤较卵巢浆液性囊腺瘤和黏液性囊腺瘤组织中HIF-1α的阳性率均明显增高(P<0.05)。卵巢浆液性囊腺癌和黏液性囊腺癌组织中HIF-1α表达的阳性率均明显高于两组卵巢良性肿瘤(P<0.05)。卵巢交界性浆液性囊腺瘤和卵巢交界性黏液性囊腺瘤两交界性肿瘤组织中，MVD值均较两良性肿瘤组明显增高(P<0.05)。卵巢浆液性囊腺癌和黏液性囊腺癌组织中MVD值均较两良性肿瘤组和两交界性肿瘤组明显增高(P<0.05)。随着卵巢癌手术-病理分期级别的增高，HIF-1α 表达的阳性率也由Ⅰ期的38.46%逐渐增高至Ⅳ期的83.33%，其中Ⅳ期较Ⅰ期的阳性率明显增高(P<0.05)。随手术-病理分期级别的增高，MVD也增高，各组间比较差异具有显著性(P<0.05)。不同组织类型及手术病理分期的卵巢上皮性肿瘤组织中，HIF-1α表达与MVD呈正相关关系(r =0.540，P<0.01)。结论：HIF-1α 与卵巢上皮性肿瘤血管形成密切相关，提示HIF-1α可能在促进卵巢上皮性肿瘤的发生、发展起到重要的作用。

关键词: α亚基, 微血管密度, 卵巢肿瘤

Abstract: Objective To detect the expressions of hypoxia inducible factor - 1α (HIF-1α) and microvesseldensity (MVD)in ovarian epithelial tumor tissues ,and investigate the effect of HIF-1α and MVD in development of ovarian epithelial tumor.Methods 237 patients with ovarian epithelial tumor were selected,including 79 cases of serous cystadenoma, 12 cases of borderline serous cystadenoma,51 cases of serous cystadenocarcinoma, 67 cases of mucinous cystadenoma,9 cases of borderline mucinous cystadenoma and 19 cases of mucous cystoadenocarcinoma.Serous cystadenocarcinoma and mucous cystoadenocarcinoma were divided into different stages according to FIGO operation-pathology,stage Ⅰ was 13 cases,stage Ⅱ 26 cases,stage Ⅲ 19 cases and stage Ⅵ 12 cases.The expressions of of HIF-1α and MVD in ovarian epithelial tumor tissues were detected with immunohistochemistry.The expressions of HIF-1α and MVD were compared in benign tumor,borderline tumor and malignant tumor,as well as in different stages of malignant tumor.The relationship between the expression of HIF-1α and MVD value was also analyzed.Results The positive expression rate of HIF-1α in borderline serous cystadenoma group was significantly increased,compared with ovarian benign tumors (serous cystadenoma and mucinous cystadenoma groups)(P<0.05).The positive expression rates of HIF-1α in ovarian serous cystadenocarcinoma and mucous cystoadenocarcinoma were significantly superior to the benign tumors(P<0.05).The expressions of MVD in borderline serous cystadenoma and borderline mucinous cystadenoma groups were significantly higher than that in ovarian benign tumors(P<0.05).The expressions of MVD in serous cystadenocarcinoma and mucous cystoadenocarcinoma groups were sinigicantly higher than those in benign tumor group and borderline tumor group(P<0.05).The positive expression rates of HIF-1α and MVD were raised up with the process of the stage of FIGO operation-pathology.And there was positive correlation between HIF- 1α positive expression and MVD (r=0.540，P<0.01).Conclusion The overexpression of HIF-1α in ovarian epithelial tumor is closely related with angiogenesis,which may play an important role in the occurrence and development of ovarian epithelial tumor.

Key words: alpha subunit, microvessel density, ovarian neoplasms

中图分类号:

R737.31

付莉，刘爱民，冯卫，王爱绘，王冰冰，杨丽晓，张茜. HIF-1α和MVD在卵巢上皮性肿瘤中的表达及意义[J]. J4, 2009, 35(2): 369-372.

FU Li，LIU Ai-min，FENG Wei，WANG Ai-hui，WANG Bing-bing，YANG Li-xiao，ZHANG Qian. Expressions of hypoxia inducible factor - 1α and microvessel density in ovarian epithelial tumor tissues and significance[J]. J4, 2009, 35(2): 369-372.

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