氯化三乙基锡对体外培养大鼠C6胶质瘤细胞的增殖抑制作用

J4 ›› 2009, Vol. 35 ›› Issue (4): 595-598.

氯化三乙基锡对体外培养大鼠C6胶质瘤细胞的增殖抑制作用

张适¹, 张悦², 毕晓颖³, 李志超³

1.北华大学基础医学院病理学教研室|吉林吉林132013；2.厦门大学附属厦门眼科中心|福建厦门361001； 3.吉林大学公共卫生学院毒理学教研室|吉林长春130021

收稿日期:2009-01-04 出版日期:2009-07-28 发布日期:2009-08-24
通讯作者: 李志超 E-mail:lizhichao@jlu.edu.cn
作者简介:张适（1970-）|男|吉林省吉林市人|副教授|医学博士|主要从事重金属生物学效应
基金资助:
国家自然科学基金资助课题（30070645）

Inhibitory effect of triethyltin chloride on proliferation of |rat C6 glioma cells in vitro

ZHANG Shi¹, ZHANG Yue², BI Xiao-Ying³, LI Zhi-Chao³

1.Department of Pathology,School of Basic Medical Sciences,Beihua University,Jilin 132013,China;2.Xiamen Eye Center,Xiamen University,Xiamen 361001,China|3.Department of Toxicology,School of Public Health,Jilin University,Changchun 130021,China

Received:2009-01-04 Online:2009-07-28 Published:2009-08-24

摘要/Abstract

摘要：

目的：探讨氯化三乙基锡（TETC）对体外培养大鼠C6胶质瘤细胞的增殖抑制作用及其机制，为胶质瘤治疗提供实验依据。方法：采用MTT法检测0.5、1.0和2.0 μmol/L TETC对大鼠C6胶质瘤细胞作用24和48 h后细胞增殖抑制率，采用Hoechest33258染色荧光显微镜观察0.5、1.0和2.0 μmol/L TETC作用48 h后 C6胶质瘤细胞核的形态学变化，采用流式细胞术(FCM)检测0.5、1.0和2.0 μmol/L> TETC作用48 h后 C6胶质瘤细胞周期和凋亡。结果：0.5、1.0和2.0 μmol/L TETC在体外可抑制C6胶质瘤细胞增殖，24 h抑制率分别为7.92％、9.51％和19.03％；48 h抑制率分别为15.62％、36.16％和41.92％，存在剂量依赖性和时间依赖性上升趋势，48 h抑制率在各剂量组间及各剂量组与对照组间比较差异有显著性（P<0.05或P<0.01）。荧光显微镜显示TETC作用C6胶质瘤细胞48 h后，细胞呈现核浓缩和碎裂的凋亡形态学改变。1.0和2.0 μmol/L TETC作用C6胶质瘤细胞48 h后，G₀/G₁期细胞比例明显高于对照组（P<0.05），S期细胞比例明显低于对照组（P<0.05
），细胞凋亡比例明显高于对照组（P<0.05）。结论：TETC对大鼠C6胶质瘤细胞有增殖抑制作用，通过细胞周期阻滞及诱导凋亡可能是TETC抑制C6胶质瘤细胞增殖的作用机制。

关键词: 三乙基锡化合物；胶质瘤细胞；细胞凋亡

Abstract:

Abstract:Objective To study the inhibitory effect of triethyltin chloride (TETC) on proliferation of rat C6 glioma cells in vitro and its mechanism and provide basis for research on TETC in treatment for glioma. Methods MTT assay was performed to determine the inhibitory rate of 0.5,1.0 and 2.0 μmol/L TETC on rat C6 glioma cells for 24 and 48 h.The changes of nucleus of C6 glioam cells treated with 0.5,1.0 and 2.0 μmol/L TETC for 48 h were observed by fluorescent microscope. Flow cytometry was used to assess the effects of 0.5,1.0 and 2.0 μmol/L TETC on cell cycle and apoptosis in rat C6 glioma cells for 48 h. Results 0.5,1.0 and 2.0 μmol/L TETC inhibited the proliferation of C6 glioma cells in vitro,and the inhibitory rates were 7.92%,9.51%,19.03% and 15.62%,36.16%,41.92% in 24 h TETC treated group and 48 h TETC treated group,respectively. The inhibitory rate of TETC on C6 glioma cells determined by MTT assay increased in a dose-dependent and time-dependent manner，and there were significant differences of the inhibitory rates at 48 h between control and various doses groups as well as between various doses groups (P<0.05 or P<0.01) . Pyknotic nucleus and nuclear fragment were shown by fluorescent microscope. When C6 glioma cells were treated with 1.0 and 2.0 μmol/LTETC for 48 h,the ratio of G₀/G₁ phase cells significantly increased compared with control group(P<0.05);while the cells at S phase were decreased(P<0.05);the apoptotic cells were significantly higher than those in control group(P<0.05).
Conclusion TETC can inhibit the proliferation of rat C6 glioma cells in vitro,the mechanism of which may be involved in cell cycle arrest and apoptosis.

Key words: triethyltin compounds;glioma cells;apoptosis

中图分类号:

R739.4

张适, 张悦, 毕晓颖, 李志超. 氯化三乙基锡对体外培养大鼠C6胶质瘤细胞的增殖抑制作用[J]. J4, 2009, 35(4): 595-598.

ZHANG Shi, ZHANG Yue, BI Xiao-Ying, LI Zhi-Chao. Inhibitory effect of triethyltin chloride on proliferation of |rat C6 glioma cells in vitro[J]. J4, 2009, 35(4): 595-598.

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