海仁藻酸致痫大鼠肾脏ERK1/2表达的检测及意义

J4 ›› 2009, Vol. 35 ›› Issue (4): 635-638.

海仁藻酸致痫大鼠肾脏ERK1/2表达的检测及意义

马宝新¹, 张丽红², 王赞¹, 吴绥生¹

1.吉林大学第一医院干部病房|吉林长春 130021;2. 吉林大学基础医学院病理生物学教育部重点实验室|吉林长春 130021

收稿日期:2008-07-23 出版日期:2009-07-28 发布日期:2009-08-24
通讯作者: 吴绥生 E-mail:Wusuisheng8888@163.com
作者简介:马宝新（1980－）|男|山东省济南市人|在读医学博士|主要从事高血压及冠心病的基础与临床研究。
基金资助:
吉林省科技厅科研基金资助课题（200705159）

Determination of ERK1/2 expression of renal cells in epilepsy rat induced by kainic acid and its significance

MA Bao-Xin¹, ZHANG Li-Gong², WANG Zan¹, WU Sui-Sheng¹

1.Department of Cadre Ward,First |Hospital,Jilin University,Changchun 130021,China;2. Key Laboratory
of Pathobiology,Ministry of Education,School of Basic Medical Sciences,Jilin
University,Changchun 130021,China

Received:2008-07-23 Online:2009-07-28 Published:2009-08-24

摘要/Abstract

摘要：

目的：研究海仁藻酸(KA)致痫大鼠肾脏细胞外信号调节激酶1/2（ERK1/2）表达情况，阐明癫痫引起肾损伤的可能机制。方法：健康雄性Wistar大鼠70只，随机分为致痫组和正常对照组，每组35只。采用立体定位仪下杏仁核内注射KA方法制备癫痫动物模型，在大鼠癫痫发作后0、2、6、12和24 h制备肾脏组织标本，采用免疫组织化学法检测正常对照组和致痫组大鼠癫痫发作后不同时间肾脏组织ERK1/2表达水平，并与对照组对比。结果：致痫组大鼠肾脏组织ERK1/2表达水平于癫痫发作后逐渐增加，6 h肾组织ERK1/2的表达达到高峰，高于癫痫发作后0 h（P<0.01），随后逐渐下降；至癫痫发作24 h，肾脏ERK1／2表达恢复至致痫0 h的表达水平（P>0.05）。致痫组大鼠肾脏HE染色显示，肾小管、肾小球均无明显形态学改变。结论：ERK1/2　活化可能在癫痫所引起的肾脏损伤中具有重要的作用，抑制ERK1/2活化对肾脏起到保护作用。

关键词: 癫痫；海仁藻酸；肾小管上皮细胞；细胞外信号调节激酶

Abstract:

Objective
To study the extracellular signal-regulated kinase 1/2(ERK1/2) expression of renal cells in epilepsy rats induced by kainic acid,and elucidate the mechanism of renal injury caused by epilepsy.Methods 70 male Wistar rats were randomly divided into two groups:epilepsy group and control group,35 rats in each group.Rat epilepsy model was prepared by injection of kainic acid into amygdala under three-dimensional positioning devices.At different time points(0,2,6,12 and 24 h) after epilepsy spasm,the renal tissue specimens were immediately prepared for immunohistochemistry.The ERK1/2 expression of rat renal cells was calculated and compared with control group.Results The ERK1/2 expression (gray value) of renal tubular epithelial cells in epilepsy rats began to increase gradually after epilepsy spasm.It reached peak at 6 h after epilepsy spasm.The ERK1/2 expression of renal tubular epithelial cells in epilepsy rats was significantly higher than at 0 h （P<0.01）,then it decreased gradually;at 24 h after spasm,the renal tissue ERK1/2 expression was as high as at 0 h （P>0.05）.There were no major pathological changes in kidney of rats with epilepsy stained with HE.Conclusion ERK1/2 activation may have important role in the renal injury caused by epilepsy.Inhibition of the activation of ERK1/2 may lead to the renal protection.

Key words: epilepsy;kainic acid;renal tubular epithelial cells;extracellular signal-regulated kinase

中图分类号:

R-332

马宝新, 张丽红, 王赞, 吴绥生. 海仁藻酸致痫大鼠肾脏ERK1/2表达的检测及意义[J]. J4, 2009, 35(4): 635-638.

MA Bao-Xin, ZHANG Li-Gong, WANG Zan, WU Sui-Sheng. Determination of ERK1/2 expression of renal cells in epilepsy rat induced by kainic acid and its significance[J]. J4, 2009, 35(4): 635-638.

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