氯化甲基汞诱导脑胶质瘤细胞凋亡的形态学变化及意义

J4 ›› 2009, Vol. 35 ›› Issue (4): 642-645.

氯化甲基汞诱导脑胶质瘤细胞凋亡的形态学变化及意义

柳影¹|陈儇¹|毕晓颖²|李志超²|袁长吉¹

1.吉林大学第一医院肿瘤中心|吉林长春 130021；2.吉林大学公共卫生学院毒理学教研室|吉林长春 130021

收稿日期:2009-03-09 出版日期:2009-07-28 发布日期:2009-08-24
通讯作者: 袁长吉 E-mail:yuanchangji@163.com
作者简介:柳影（1970-）|女|吉林省长春市人|副主任医师|在读医学博士|主要从事实体肿瘤基础和临床研究。
基金资助:
国家自然科学基金资助课题（30070646，39770655）

Morphological changes of apoptotic brain neuroglioma cells induced 　with |methyl-mercuric chloride and significances

LIU Ying¹, CHEN Xuan¹, BI Xiao-Ying², LI Zhi-Chao², YUAN Chang-Ji¹

1.Tumor Center|First Hospital, Jilin University,Changchun 130021, China;2.Department of Toxicology, School of Public Health, Jilin University, Changchun. 130021, China

Received:2009-03-09 Online:2009-07-28 Published:2009-08-24

摘要/Abstract

摘要：

目的：通过观察氯化甲基汞(MMC)诱导大鼠脑胶质瘤细胞凋亡的形态学改变，探讨MMC的抗脑胶质瘤作用。方法：制备大鼠脑胶质瘤动物模型，将造模成功的大鼠随机分为对照组和实验组，实验组大鼠注射C6脑胶质瘤细胞1周后，每天灌胃给予10 mg·kg^-1MMC，连续5 d；对照组给予相同体积的生理盐水。除自然死亡外，其余存活大鼠在第24天全部处死，取大鼠脑组织，光镜及透射电镜观察其病理组织学改变。结果：大体病理显示，实验组大鼠肿瘤体积明显小于对照组；光镜下显示，实验组大鼠C6胶质瘤细胞生长密度低于对照组，并可见细胞体积变小、核固缩深染的凋亡形态学改变细胞。透射电镜显示，实验组大鼠肿瘤细胞发生核固缩、染色质周边化、核断裂、胞浆空泡变性等改变；对照组大鼠肿瘤细胞核体积增大，外形不规则，核常染色质为主，核仁突出。结论：MMC具有抑制大鼠体内C6胶质瘤增殖的作用，其机制可能是通过诱导胶质瘤细胞凋亡实现。

关键词: 脑胶质瘤；氯化甲基汞；细胞凋亡

Abstract:

Objective To study the anti-neuroglioma effect of methyl-mercuric chloride(MMC) by observing the morphological changes of apoptotic neuroglioma cells induced with MMC in rats with brain neuroglioma.Methods The rat models of neuroglioma were established,and divided into two groups. The rats in experimental group were lavaged with MMC 1 week after injected with C6 glioma cells, 10 mg·kg^-1every day,the rats in control group were treated with sodium chloride at the same dose. 24 d after inoculation all rats were sacrificed except natural death, the brain tissues were obtained ,and the pathohistological changes were observed under light microscope and transmission electron microscope.Results The macropathological result showed that the tumor volume in experimental group was smaller than that in control group.Under light microscope,in experimental group the growth density of C6 ghioma cells was lower than that in control group,and the apoptotic cells with smaller volume and karyopyknosis were found.The result of transmission electron microscope showed that in experimental group,the glioma cells had some changes such as karyopyknosis,chromoplasm margination,nuclear fragmentation and vacuolar degeneration and so on.Conclusion MMC has inhibitory effect on the proliferation of C6 glioma in rats in vivo,its mechanism may be related to inducing the apoptosis of neuroglioma cells.

Key words: brain neuroglioma, methyl-mercuric chloride, apoptosis

中图分类号:

R739.41

柳影, 陈儇, 毕晓颖, 李志超, 袁长吉. 氯化甲基汞诱导脑胶质瘤细胞凋亡的形态学变化及意义[J]. J4, 2009, 35(4): 642-645.

LIU Ying, CHEN Xuan, BI Xiao-Ying, LI Zhi-Chao, YUAN Chang-Ji. Morphological changes of apoptotic brain neuroglioma cells induced 　with |methyl-mercuric chloride and significances[J]. J4, 2009, 35(4): 642-645.