羊布鲁氏菌外膜蛋白质的组成鉴定

J4 ›› 2009, Vol. 35 ›› Issue (5): 805-811.

羊布鲁氏菌外膜蛋白质的组成鉴定

曲勍^1,2, 王玉飞¹, 徐杰¹, 钟志军¹, 乔凤², 杜昕颖¹, 汪舟佳¹, 黄留玉¹, 于雅琴², 陈泽良¹

1. 军事医学科学院疾病预防所|北京 100071；2. 吉林大学公共卫生学院　人类基因组医学研究中心|吉林长春 130021

收稿日期:2009-06-01 出版日期:2009-09-28 发布日期:2009-09-28
通讯作者: 于雅琴 E-mail:yuyaqin5540@sina.com
作者简介:曲勍（1980-）|女|吉林省长春市人|在读医学博士|主要从事布鲁菌致病机制的研究。
基金资助:
国家高技术研究发展计划(863)资助课题（2007AA02Z412）；“十一五”重大专项子课题资助（2008ZX1004-015）

Identification of outer membrane proteins of Brucella Melitensis

QU Qing^,,2, WANG Yu-Fei¹, XU Jie¹, ZHONG Zhi-Jun¹, QIAO Feng², DU Xin-Ying¹, WANG Zhou-Jia¹, HUANG Liu-Yu¹, YU Ya-Qin¹, CHEN Ze-Liang¹

1.Institute of Disease Control and Prevention,Academy of Military Medical Science,Beijing 100071,China;2.School of Public Health,Research Center for Genomic Medicine,Jilin University,Changchun 130021,China

Received:2009-06-01 Online:2009-09-28 Published:2009-09-28

摘要/Abstract

摘要：

目的：通过蛋白质组技术阐明羊布鲁氏菌外膜蛋白的主要组成。方法：提取并纯化羊布鲁氏菌的外膜蛋白，应用双向电泳技术进行分离，选取双向电泳图上主要的蛋白点进行质谱分析，同时主要蛋白质点的肽指纹图谱利用Mascot在NCBInr蛋白数据库中进行检索。结果：共鉴定到67个蛋白点，主要外膜蛋白为Omp25和Omp31，其他外膜蛋白如Imp、Frp、GroEL等，功能涉及物质运输、能量代谢、应激等。结论：对主要外膜蛋白Omp25和Omp31的识别与分析不仅有助于对布鲁氏菌致病机制的研究，而且为布鲁氏菌病的疫苗研制提供靶标蛋白。

关键词: 布鲁氏菌；细菌外膜蛋白质类；双向电泳

Abstract:

Abstract:Objective To identify the major outer membrane proteins(OMPs) of Brucella Melitensis with proteome analysis. Methods The OMPs were extracted from the culture of Brucella Ｍelitensis and then the two dimension electrophoresis was used to separate the OMPs.By means of matrix-assisted laser descorption/ionization time of light mass spectrometry (MALDI-TOF-MS),the OMPs were identified.Peptide mass fingerprints were searched in the NCBInr database using the Program Mascot.Results Sixty seven protein spots were identified,in which Omp25 and Omp31 were identified as two main OMPs and other OMPs,such as Imp,Frp and GroEL were found.The functions of identified OMPs included thymidylate kinase,protein precursor,NADH dehydrogenase subunit,cytosol aminopeptidase,etc.Conclusion Identification and analysis of Omp25 and Omp31 provides an important clue for understanding the molecular mechanism of Brucella Melitensis and developing the vaccine to prevent
Brucella attack.

Key words: brucella;bacterial outer membrane proteins;two-dimensional electrophoresis (2-DE)

中图分类号:

R378.5

曲勍, 王玉飞, 徐杰, 钟志军, 乔凤, 杜昕颖, 汪舟佳, 黄留玉, 于雅琴, 陈泽良. 羊布鲁氏菌外膜蛋白质的组成鉴定[J]. J4, 2009, 35(5): 805-811.

QU Qing, WANG Yu-Fei, XU Jie, ZHONG Zhi-Jun, QIAO Feng, DU Xin-Ying, WANG Zhou-Jia, HUANG Liu-Yu, YU Ya-Qin, CHEN Ze-Liang. Identification of outer membrane proteins of Brucella Melitensis[J]. J4, 2009, 35(5): 805-811.