两种剂量野百合碱诱导的大鼠肺动脉高压模型肺组织病理变化比较

J4 ›› 2009, Vol. 35 ›› Issue (5): 870-873.

两种剂量野百合碱诱导的大鼠肺动脉高压模型肺组织病理变化比较

赵科研, 孙江滨, 柳克祥, 吴慧颖, 李博

吉林大学第二医院心血管外科|吉林长春 130041

收稿日期:2009-03-10 出版日期:2009-09-28 发布日期:2009-09-28
通讯作者: 柳克祥 E-mail:kxliu64@hotmail.com
作者简介:赵科研(1975－)|男|辽宁省葫芦岛市人|主治医师|在读医学博士|主要从事器官、细胞移植及复杂先心病治疗。
基金资助:
吉林省科技厅科技发展计划资助课题（200705196）

Comparison of pathological changes of lung tissue in rat pulmonary arterial hypertension model induced by two different doses of monocrotaline

ZHAO Ke-Xing, SUN Jiang-Bin, LIU Ke-Xiang, WU Hui-Ying, LI Bo

Received:2009-03-10 Online:2009-09-28 Published:2009-09-28

摘要/Abstract

摘要：

目的: 建立野百合碱(MCT)诱导的肺动脉高压动物模型，观察肺组织的病理变化，并比较50和60 mg·kg-1MCT两种剂量诱导不同变化。方法: 70只雄性Wistar大鼠随机分为对照组（n=10）、50 mg·kg^-1MCT组（n=30)、60 mg·kg^-1MCT组(n=30)。腹腔注射MCT（对照组注射等量生理盐水），在2周及4周后检测右心室收缩压(RVSP)、RV/(LV+S)重量比值，肺组织苏木素-伊红染色和地衣红染色，观察肺组织的病理改变、肺小动脉中膜厚度百分比。50 mg·kg^-1组和60 mg·kg^-1组各10只记录大鼠自然死亡天数。结果:大鼠经过MCT诱导后，50 mg·kg^-1组2周和4周时与对照组比较：RVSP增高［(36.6±5.1)mmHg，(39.1±7.0)mmHg 和 (26.1±3.8)mmHg，P<0.01］；RV/(LV+VS)重量比增加［(0.289　4±0.021　7)，(0.409　4±0.125　6)和(0.247　3±0.019　3)，P<0.01］。60 mg·kg^-1组2周和4周时与对照组比较:RVSP升高［(33.8±5.5)mmHg，(46.6±12.6)mmHg和(26.1±3.8)mmHg，P<0.01］；RV/(LV+VS)重量比增加［(0.308 2±0.058 4),(0.417 5±0.103 7)和(0.247 3±0.019 3),P<0.01］，但两个剂量组之间在2周和4周时比较均无显著性差异。4周时50 mg·kg^-1和60 mg·kg^-1MCT组肺小动脉中膜增厚，厚度百分比分别为（21.3±1.9）%和（23.4±2.3）%，高于对照组的（11.3±1.2）%(P<0.01)。60 mg·kg^-1MCT组大鼠47 d死亡50％，63 d全部死亡；而50 mg·kg^-1诱导后47 d未见死亡，63 d死亡30%。结论: 50和60 mg·kg^-1野百合碱均可诱导建立肺动脉高压模型，病理变化未见明显不同，但前者较后者有较长的生存期。

关键词: 野百合碱;模型,动物;高血压, 肺性

Abstract:

Abstract:Objective To establish rat models with pulmonary artery hypertension induced by monocrotaline(MCT) and to observe the pathological changes of lung tissue.Methods Seventy male Wistar rats were randomly devided into three group:control group(n=10),50 mg·kg^-1 MCT group(n=30),60 mg·kg^-1 MCT group (n=30).At two weeks and four weeks after injected intraperitoneally with MCT(injected intraperitoneally with equal normal saline in control group),the right ventricular systolic pressure(RVSP)and right ventricle weight/left ventricle+septum weight ［RV/(LV+S)］ ratio were measured.Hematoxylin -eosin staining and orcein technique were used to observe the pathological changes of lung tissue and pulmonary arterioles’medial thickness.Results Two weeks or four weeks after MCT administration,RVSP in 50 mg·kg^-1 MCT group was higher than that in control group（respectively 36.6 mmHg±5.1 mmHg，39.1 mmHg±7.0 mmHg versus 26.1 mmHg±3.8 mmHg，both P<0.01）,and RV/(LV+VS) weight ratio increased greatly（respectively 0.289 4±0.021 7，0.409 4±0.125 6 versus 0.247 3±0.019 3,both P<0.01）,so were the results in 60 mg·kg^-1 MCT group compared with control group:RVSP respectively 33.8 mmHg±5.5 mmHg,46.6 mmHg±12.6 mmHg versus 26.1 mmHg±3.8 mmHg，both P<0.01;RV/(LV+VS) weight ratio,respectively 0.308 2±0.058 4,0.417 5±0.103 7 versus 0.247 3±0.019 3,both P<0.01.There was no significant difference of RVSP and RV/(LV+VS) weight ratio between two different doses of MCT groups. The pulmonary arterioles were markedly thickened in both 50 and 60 mg·kg-1 MCT groups compared with control group（21.3%±1.9%,23.4%±2.3% versus 11.3%±1.2%,respectively P<0.01).The rats in 60 mg·kg^-1 MCT group were half dead by the day 47 and none survived by the day 63,yet none of rats in 50 mg·kg^-1 MCT group was dead by the day 47 and seventy percent survived by the day 63.Conclusion Both 50 and 60 mg·kg^-1 MCT can induce pulmonary artery hypertension and there were no pathological differences between them，but the survival of rats treated by the former dose is longer than that by the latter one.

Key words: monocrotaline;models,animal;hypertension,pulmonary

中图分类号:

R541.5

赵科研, 孙江滨, 柳克祥, 吴慧颖, 李博. 两种剂量野百合碱诱导的大鼠肺动脉高压模型肺组织病理变化比较[J]. J4, 2009, 35(5): 870-873.

ZHAO Ke-Xing, SUN Jiang-Bin, LIU Ke-Xiang, WU Hui-Ying, LI Bo. Comparison of pathological changes of lung tissue in rat pulmonary arterial hypertension model induced by two different doses of monocrotaline[J]. J4, 2009, 35(5): 870-873.

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