山葡萄籽提取物中总多酚的含量测定

J4 ›› 2009, Vol. 35 ›› Issue (5): 877-879.

山葡萄籽提取物中总多酚的含量测定

贾荣¹, 倪海镜¹, 赵春芳², 戴维群¹

1.吉林大学中日联谊医院药剂科|吉林长春 130033；2.吉林大学药学院药物分析教研室|吉林长春 130021

收稿日期:2009-03-03 出版日期:2009-09-28 发布日期:2009-09-28
通讯作者: 倪海镜 E-mail:nihaij9966@126.com
作者简介:贾荣（1982-）|女|山西省原平市人|在读医学硕士|主要从事临床药学的研究。
基金资助:
吉林省科技厅科研基金资助课题（200302-05）

Determination of totol polyphenols in Vitis amurensis seed extract

JIA Rong¹, NI Hai-Jing¹, ZHAO Chun-Fang², DAI Wei-Qun¹

1.Department of Pharmacy Service|Chain-Japan Union Hospital|Jinlin University|Changchun 130033|China；2.Department of Pharmaceutical Analytics|School of Pharmacy|Jilin University|Changchun 130021|China

Received:2009-03-03 Online:2009-09-28 Published:2009-09-28

摘要/Abstract

摘要：

目的：建立山葡萄籽提取物中总多酚含量测定的方法。方法：以没食子酸为对照品，采用Folin- Ciocalteus比色法最适宜条件对山葡萄籽提取物中总多酚含量进行测定。结果：没食子酸对照品在浓度为0.002～0.010 g·L^-1范围内，与吸光度成良好的线性关系（Y=86.243X+0.007,r=0.999　6）；精密度试验RSD为0.14%；稳定性实验RSD为1.77%；重复性实验RSD为1.44%；平均回收率为102.00%，RSD为1.37%。结论：该法灵敏度高，重复性好，简便快捷。

关键词: 山葡萄籽；总多酚；没食子酸；比色法

Abstract:

Abstract:Objective To establish the determination method of total polyphenols in vitis amurensis seed extract.Methods Using gallic acid as reference substance，the content of total polyphenols in Vitis amurensis seed extract was determined by the optimum conditions of Folin-Ciocalteu chromatometry.Results There was a good linearity between gallic acid concentration and absorbance in the range 0.002-0.010 g·L^-1（Y=86.243X+0.007,r=0.9996）；the precision RSD was 0.14%；the stability RSD was 1.77%； the repeatability RSD was 1.44%； the average recovery rate was 102.00%（RSD=1.37%）.Conclusion This method is simple，fast and has high sensitivity and good reproducibility.

Key words: Vitis amurensis seeds；total polyphenols；gallic acid； chromatometry

中图分类号:

R284.2

贾荣, 倪海镜, 赵春芳, 戴维群. 山葡萄籽提取物中总多酚的含量测定[J]. J4, 2009, 35(5): 877-879.

JIA Rong, NI Hai-Jing, ZHAO Chun-Fang, DAI Wei-Qun. Determination of totol polyphenols in Vitis amurensis seed extract[J]. J4, 2009, 35(5): 877-879.