关键词: 人纤溶酶；抗栓；真核表达载体；重组质粒

Abstract:

Abstract:Objective To construct the eukaryotic expression vector of human fibrinolysin for further  study on anti-thrombus and thrombolysis. Methods  Human fibrinolysin gene was amplified  from human fetal liver tissue with PCR，and was cloned into  eukaryotic expression plasmid pCI-neo and then sequenced.After the correct identification by sequencing,the eukaryotic expression recombinant plasmid of human fibrinolysin was constructed and identified with PCR and enzyme digestion.Results The PCR amplification product of fibrinolysin gene was 1 740 bp.The result of sequencing was the same as that registered in GenBank.The results of PCR and enzyme digestion showed that the recombinant eukaryotic expression plasmid had  correct codogenic gene fragment.Conclusion The eukaryotic expression recombinant plasmid of human fibrinolysin gene is successfully constructed and identified.

Key words:  human fibrinolysin;anti-thrombus;eukaryotic expression vector;recombinant plasmid