J4 ›› 2009, Vol. 35 ›› Issue (6): 971-974.

• 基础研究 •    下一篇

c-myc基因与辐射诱发小鼠胸腺淋巴瘤的关联性分析

于雷, 刘永哲, 孙世龙, 方芳, 巩宏伟, 陈强, 鞠桂芝   

  1. 吉林大学公共卫生学院 卫生部放射生物学重点实验室|吉林 长春 130021
  • 收稿日期:2009-08-06 出版日期:2009-11-28 发布日期:2009-11-28
  • 通讯作者: 鞠桂芝 E-mail:jugz@jlu.edu.cn
  • 作者简介:于 雷 (1977-)|男|吉林省长春市人|讲师|在读医学博士|主要从事辐射肿瘤学研究。
  • 基金资助:

    国家自然科学基金资助课题(30670630);吉林大学研究生创新基金资助课题(20091032)

Analysis on relevance between c-myc gene and thymic lymphomas of mice induced by ionizing radiation

YU Lei, LIU Yong-Zhe, SUN Shi-Long, FANG Fang, GONG Hong-Wei, CHEN Qiang, JU Gui-Zhi   

  1. Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University| Changchun 130021,China
  • Received:2009-08-06 Online:2009-11-28 Published:2009-11-28

摘要:

目的:研究辐射诱发小鼠胸腺淋巴瘤c-myc基因表达的变化,为阐明辐射致癌机制提供理论依据。方法:采用X射线(剂量率0.287 Gy?min-1,总剂量7 Gy)照射BALB/c小鼠,建立小鼠胸腺淋巴瘤模型,6个月后处死小鼠,取辐射诱发小鼠胸腺淋巴瘤和正常胸腺,分别提取总RNA、合成cDNA和提取总蛋白,采用实时定量PCR方法和Western blotting方法分别检测辐射诱发胸腺淋巴瘤和正常胸腺组织c-myc基因mRNA和蛋白表达。结果:经实时定量PCR检测,胸腺淋巴瘤和正常胸腺组织c-myc基因mRNA相对表达量分别为9.16±4.66和1.16±0.54,两组比较差异有显著性(P<0.01);Western blotting检测结果显示,胸腺淋巴瘤c-myc基因蛋白表达较正常胸腺组织明显增高。结论:c-myc基因与辐射诱发
小鼠胸腺淋巴瘤有关联,可能是辐射致癌的易感基因。

关键词: 胸腺肿瘤;淋巴瘤;基因, myc;实时定量PCR;电离辐射

Abstract:

Abstract:Objective To study the change of c-myc gene expression in thymic lymphomas of mice induced by ionizing radiation and provide theoretical basis for     mechanism of radiation carcinogenesis.Methods The thymic lymphomas models of BALB/c mice were made by   X-ray(dose rate:0.287 Gy?min-1;total dose:7 Gy) exposuring,6 months later they were killed,then total RNA  and  total protein were extracted  from thymic lymphomas a
nd normal thymus tissue and cDNA was synthesized, the expressions of c-myc mRNA and protein in thymic lymphomas induced by ionizing radiation and normal thymus were detected by quantitative real-time PCR and Western blotting respectively.Results The expression of c-myc mRNA  in thymic lymphomas(9.16±4.66) was higher than that in normal thymus tissue(1.16±0.54)(P<0.01). The expression of c-myc protein in thymic lymphomas was higher than that in normal thymus tissue. Conclusion There is relevance between c-myc gene and thymic lymphomas induced by ionizing radiation,c-myc gene might be susceptibility gene of radiation carcinogenesis.

Key words: thymic neoplasms;lymphoma;genes, myc;quantitative real-time PCR;ionizing radiation

中图分类号: 

  • Q345