关键词: HMGA2蛋白；真核细胞；载体构建；转染

Abstract:

Abstract:Objective　 To construct the recombinant eukaryotic expression vector of  HMGA2 gene and observe its expression in eukaryotic cells,and laid a theoretical foundation for further study of　HMGA2 gene function.Methods　HMGA2 gene full-length cDNA from human breast epithelial cell HBL-100 was amplified by RT-PCR;after double-digestion and gal recovery HMGA2 gene was cloned into FP-C1 eukaryotic expression vector.After eukaryotic expression vector YFP-C1-HMGA2 was transfected into human prostate cancer cells,and the correctness of constructed YFP-C1-HMGA2 was certificated by RT-PCR.Results　HMGA2 gene full-length cDNA of 351 bp was obtained by RT-PCR.After the cloning of YFP-C1 eukaryotic expression vector,double enzyme digestion and sequencing analysis,YFP-C1-HMGA2 eukaryotic expression vector was confirmed to be successful.And it could express　 in　eukaryotic cells.Conclusion　 HMGA2 gene is cloned successfully,and YFP-C1-HMGA2 eukaryotic　expression vector is constructed successfully.

Key words: HMGA2 protein;eukaryotic cell;vector construction;transfection