局部应用辛伐他汀对拔牙窝内BMP-2 mRNA表达的影响及意义

J4 ›› 2010, Vol. 36 ›› Issue (5): 851-853.

局部应用辛伐他汀对拔牙窝内BMP-2 mRNA表达的影响及意义

吴健¹, 钱明¹, 吴哲¹, 马冬丽¹, 孙宏晨²

1. 吉林大学口腔医院修复科|吉林长春 130021；2.吉林大学口腔医院病理科|吉林长春 130021

收稿日期:2010-02-08 出版日期:2010-09-28 发布日期:2010-09-28
通讯作者: 吴哲（Tel:0431-88796055,E-mail:lcwztt@sina.com） E-mail:lcwztt@sina.com
作者简介:吴健（1974-）|男|吉林省吉林市人|讲师|医学硕士|主要从事口腔修复学基础及临床研究。
基金资助:
国家自然科学基金资助课题 (30872912)

Effects of simvastatin on BMP-2 mRNA expression in alveolar bone following tooth extraction and significance

WU Jian¹, QIAN Meng¹, WU Zhe¹, MA Dong-Li¹, SUN Hong-Chen²

1.Department of Prosthodontics,Stomatology Hosptial,Jilin University,Changchun 130021,China;2.Department of Pathology,Stomatology Hosptial,Jilin University,Changchun 130021,China

Received:2010-02-08 Online:2010-09-28 Published:2010-09-28

摘要/Abstract

摘要：

目的：探讨局部应用辛伐他汀对拔牙窝内骨形成蛋白(BMP)〖KG-*3〗-2　mRNA表达的影响，阐明其作用机制。方法：选用健康雄性 Wistar大鼠56只,随机分为实验组28只和对照组28只,拔除右下颌中切牙后,实验组即刻植入载辛伐他汀聚乳酸-羟基乙酸共聚物(PLGA)支架材料,对照组植入单纯 PLGA支架材料作为对照,于术后5 d、1周、2周和4周分别处死大鼠,设计针对BMP-2 mRNA的寡核苷酸探针,采用原位杂交技术检测拔牙窝 BMP-2 mRNA的表达。结果：术后5 d，实验组和照组均未见有BMP-2 mRNA阳性表达细胞；术后1周,实验组植入材料和牙槽窝骨壁之间的间充质细胞内可见染色较深的阳性细胞，而对照组偶见染色较浅的阳性细胞；术后2周，实验组在植入材料和牙槽窝骨壁之间的间充质细胞内可见较多颗粒清晰的阳性细胞，新形成的类骨质表面成骨细胞内也有BMP-2 mRNA阳性表达，阳性细胞数明显多于对照组；术后4周，实验组和对照组均可见BMP-2 mRNA阳性表达细胞。术后1周、2周、4周实验组BMP-2 mRNA阳性细胞数明显多于对照组（P<0.05）。结论：局部应用辛伐他汀可通过增加BMP-2 mRNA的表达促进拔牙窝骨损伤的愈合。

关键词: 辛伐他汀;牙槽骨;BMP-2 mRNA

Abstract:

Abstract:Objective
To investigate the effect of local application of simvastatin on the expression of bone morphogenetic protein-2(BMP-2) in the tooth socket following tooth extraction and discuss its mechanism. Methods Fifty-eight male Wistar rats were divided into experimental group and control group (n=28). PLGA was immediately implanted with or without simvastatin into extraction sockets of the mandibular incisors. Oligonucleotide probe specific to BMP-2 mRNA was designed,and hybridization technique was conducted 5 d,1 week,2 weeks and 4 weeks after implantation.Results There were no positive cells in both experimental group and control group 5 d after implantation. There were deep-dying positive stromal cells between the implanted material and the wall of tooth socket in experimental group,and a few light-dying positive cells could be seen in control group 1 week after implantation. There were many positive cells with clear particles in the stromal cells between the implanted material and the wall of tooth socket,and there were also BMP-2 mRNA positively expressed cells on the surface of newly-formed osteoid in two groups,and the number of the positive cells in experimental group was higher than that in control group 2 weeks after implantation. There were BMP-2 mRNA positive cells in both the experimental group and the control group 4 weeks after implantation. The number of BMP-2 positive cells in experimental group was significantly higher than that in control group 1,2,4 weeks after implantation (P<0.05). Conclusion Topical application of simvastain could promote alveolar bone repair by up-regulating BMP-2 mRNA expression.

Key words: simvastatin;　 alveolar bone;　BMP-2 mRNA

中图分类号:

R782.05

吴健, 钱明, 吴哲, 马冬丽, 孙宏晨. 局部应用辛伐他汀对拔牙窝内BMP-2 mRNA表达的影响及意义[J]. J4, 2010, 36(5): 851-853.

WU Jian, QIAN Meng, WU Zhe, MA Dong-Li, SUN Hong-Chen. Effects of simvastatin on BMP-2 mRNA expression in alveolar bone following tooth extraction and significance[J]. J4, 2010, 36(5): 851-853.