关键词: 心肌纤维化；转录因子；大鼠；Wistar

Abstract:

Abstract:Objective
To investigate the expression and role of NF-κB in  rat's  with myocardial ischemic fibrosis,and  illuminate the relations between  NF-κB and  occurrence and development of myocardial ischemic fibrosis. Mthods 20 male Wistar rats were  randomly divided into  control group，model group(12 h,72 h and 1 week)(n=5)， Except control group,all the rats in the other groups were treated with isoproterenol hydrochloride(2 mg?kg-1).Conventional  staining,immunohistochemical staining and Masson staining were used to analyze expression of NF-κB protein in heart tissues. Results The results of HE staining showed the myocardium tissue had been a good order,the clear nuclear arms,no cell swollen. The mycardium presented many punctual necrosis at 12 h. The myocardium appeared  clear boundary  and multi-radiations  necrosis stove at 1 week. The results of Masson staining showed the myocardium had only a small number of collagen in normal control group,the expression of collagen was  obviously increased at 12 h(P<0.01) and reached the arrived  peak at 1 week. The results of NF-κB protein expression showed there was positive matter of buff or yellow. The NF-κB expression was  increased obviously at 12 h and reached the  peak at 1 week(P<0.05). Conclusion Along with,The NF-κB expression is increased obviously which indicats that NF-κB is involved in the occurrence and development of myocardial ischenic frbrosis in rats.with the prolongation of Iso injection time.

Key words: myocardial fibrosis, transcription factor;rats,wistar