氯化甲基汞对发育阶段大鼠海马组织蛋白激酶C活性的影响

J4 ›› 2010, Vol. 36 ›› Issue (5): 922-925.

氯化甲基汞对发育阶段大鼠海马组织蛋白激酶C活性的影响

郭杰¹|王铁君¹|张雯艳²|邢程³|李志超⁴|管清香⁵

1.吉林大学第二医院放疗科,吉林长春 130021； 2.吉林省妇幼保健医院|吉林长春 130021；3. 吉林省卫生厅|吉林长春 130061；4.吉林大学公共卫生学院卫生毒理学教研室|吉林长春 130021；5.吉林大学天然药物化学教研室,吉林长春 130021

收稿日期:2010-04-07 出版日期:2010-09-28 发布日期:2010-09-28
通讯作者: 管清香（Tel:0431-85619662，E-mail:guanqx@jlu.edu.cn） E-mail:guanqx@jlu.edu.cn
作者简介:郭杰(1973-),女,吉林省长春市人,讲师,医学博士,主要从事重金属神经毒作用机制研究。
基金资助:
吉林省卫生厅资助课题（3D5097923427）；吉林大学基本科研业务费专项基金资助课题（421030140427）；吉林大学青年教师基金资助课题(419070210048)

Effects of chronic methylmercury chloride exposure on activityof protein kinase C in rat hippocampus in developmental stage

GUO Jie¹, WANG Tie-Jun¹, ZHANG Wen-Yan², XING Cheng³, LI Zhi-Chao⁴, GUAN Qing-Xiang⁵

1.Department of Radiotherapy,Second Hospital,Jilin Uninversity,Changchun 130021,China;2. Maternal and Child Health Hospital of Jilin Province,Changchun 130021,China;3. Health Department of Jilin Province,Changchun 130061,China;4. Department of Health Toxicology, School of Public Health,Changchun 130021,China;5.Department of Natural Pharmaceutical Chemistry,School of Pharmacy,Jilin Uninversity,Changchun 130021,China

Received:2010-04-07 Online:2010-09-28 Published:2010-09-28

摘要/Abstract

摘要：

目的:阐明氯化甲基汞(MMC)致脑发育损伤与海马组织蛋白激酶C（PKC）活性变化之间的关系，探讨MMC致脑发育损伤机制。方法:选用Wistar雌性大鼠120只，随机分为3个MMC剂量实验组和1个对照组，每组30只，实验组母鼠从妊娠前90 d 至仔鼠出生后30 d 连续喂饲含有不同剂量MMC (0.75,1.50和3.00 mg/kg ) 的普通饲料,取其生后(PND) 3 、7 、14 、21 和30 d 仔鼠海马组织提取胞浆和胞膜PKC。采用改良Takai 法观察MMC 对发育大鼠海马PKC 活性的影响。结果:随着脑汞含量的增加,仔鼠实验组和对照组海马组织胞膜胞浆PKC的活性随仔鼠生后时间的延长，逐渐增高，生后30 d达峰值。1.50 mg/kg 剂量组生后21和30 d仔鼠以及3.00 mg?kg-1剂量组仔鼠海马组织胞膜和胞浆PKC活性较对照组明显增高（P<0.05）。结论:长期接触低剂量MMC可引起发育阶段大鼠海马组织胞浆及胞膜PKC活性升高。MMC可能通过影响大鼠脑发育过程中PKC 活性介导其神经毒性作用。

关键词: 甲基汞化合物/ 毒性;蛋白激酶C;汞中毒,神经系统

Abstract:

Abstract:Objective To study the effects of methylmercury chloride (MMC) on protein kinase C ( PKC) activity in rat developing hippocampus. Methods The animals in experimental groups were fed with standard rat chow including 0.75 mg/kg MMC,1.5 mg/kgMMC and 3.0 mg?kg-1 MMC respectively for 90 d before gestation to 30 d post parturition. Hippocampus of pups from each group on postnatal days ( PND) 3,7,17,21 and 30 were dissected. All the samples were separated into cytosol and membrane subcellular fractions and assayed for PKC activity by the improved Takai’s method. Results The membrane and cytosolic PKC activities of pup hippocampus in experimental groups were significantly higher than those in control group.The PKC activities of rats in 3.0 mg/kg MMC group and PND 21,30 in 1.5 mg?kg-1 MMC group were significantly increased compared with control group (P<0.05).Conclusion The damage of the rat developing hippocampus by chronic exposure to MMC is possibly correlative with the enhanced effect of MMC on PKC activity.

Key words: methylmercury compounds/ toxicity;protein kinase C;mercury poisoning,nervous system

中图分类号:

R135.13

郭杰, 王铁君, 张雯艳, 邢程, 李志超, 管清香. 氯化甲基汞对发育阶段大鼠海马组织蛋白激酶C活性的影响[J]. J4, 2010, 36(5): 922-925.

GUO Jie, WANG Tie-Jun, ZHANG Wen-Yan, XING Cheng, LI Zhi-Chao, GUAN Qing-Xiang. Effects of chronic methylmercury chloride exposure on activityof protein kinase C in rat hippocampus in developmental stage[J]. J4, 2010, 36(5): 922-925.