20(S)-原人参二醇对体外培养siha细胞自噬的影响

J4 ›› 2010, Vol. 36 ›› Issue (6): 1055-1058.

20(S)-原人参二醇对体外培养siha细胞自噬的影响

许多¹|赵丽晶¹|程宏¹|郭亚雄¹|韩向北¹|鲁育铭¹|赵丽娟¹|董妍²

1. 吉林大学基础医学院病理生理学教研室|吉林长春 130021；2. 美国杜兰大学细胞生物学教研室|美国新奥尔良LA70062

收稿日期:2010-06-14 出版日期:2010-11-28 发布日期:2010-11-28
通讯作者: 赵丽娟( Tel：0431-85619485，E-mail：titan2003@sina.com.cn);董妍( Tel：001-4007128899-8991088195，E-mail：drdong71@gmail.com) E-mail:titan2003@sina.com.cn;drdong71@gmail.com
作者简介:许多（1984-）|男|吉林省长春市人|医学硕士|主要从事肿瘤防治研究。
基金资助:
吉林省人民政府人才开发基金资助课题(2006JD02)

Effect of 20(S)〖KG-*3〗-proto-panaxdiol on autophag

XU Duo¹,ZHAO Li-jing¹,CHENG Hong¹,GUO Ya-xiong¹,HAN Xiang-bei¹,LU Yu-ming¹,ZHAO Li-juan¹,DONG Yan²

1. Department of Pathophysiology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;2. Department of Structural and Cellular Biology,Tulan University,New Orleans LA 70062,USA

Received:2010-06-14 Online:2010-11-28 Published:2010-11-28

摘要/Abstract

摘要：

目的：观察20(S)-原人参二醇（PPD）对人子宫颈癌siha细胞生长的抑制作用及其对自噬基因Beclin 1及MAP1-LC3转录和表达的影响，探讨PPD抑制siha细胞增殖的机制。方法：体外培养人宫颈癌siha细胞分为阴性对照组（乙醇）、阳性对照组（40 μmol·L^-1顺铂）及PPD 10、20和40 μg·L^-1组，分别用乙醇、顺铂和PPD处理细胞24、48、72和96h后，MTT法检测siha细胞增殖抑制率，RT-PCR、Western blotting及免疫细胞化学染色法观察各组siha细胞自噬相关基因Beclin 1及MAP1-LC3的转录及蛋白表达情况。结果：与对照组比较，PPD 10、20和40 μg·L^-1组细胞增殖抑制率明显增加（P<0.05），且随时间延长和剂量的增加而逐渐提高，自噬相关基因Beclin 1及MAP1-LC3的转录水平及蛋白表达水平明显上调（P<0.01）。结论：PPD可抑制体外培养siha细胞增殖，诱导siha细胞发生自噬。其机制可能与上调自噬相关基因Beclin 1及MAP1-LC3表达有关。

关键词: 20S-原人参二醇;siha细胞；自噬；Beclin 1；MAP1-LC3

Abstract:

To investigate the inhibitory effect of 20(S)-proto-panaxdiol（PPD）on the growth of siha cells in vitro and detect the transcription and expressions of autophagy related gene Beclin 1 and MAP1-LC3,and clarify the mechanism of its suppressing effect on siha cell proliferation. Methods The siha cells cultivated in vitro were treated with alcohol,40 μmol·L^-1 platinol (Pt),10 μg·L^-1 PPD,20 μg·L^-1 PPD,40 μg·L^-1 PPD for 24,48,72 and 96 h, respectively. The inhibitory rate of cell proliferation in each group was detected by MTT,the transcription and expressions of autophagy related gene Beclin 1 and MAP1-LC3 were analyzed by RT-PCR,Western blotting and immunocytochemical staining. Results After treated with 10,20 and 40 μg·L^-1 PPD,the inhibitory rates of siha cells were significantly increased compared with control group(P<0.05),and the inhibitory rate was increased gradually with the time prolongation of time and the increase of dose. The transcription and expressions of autophagy related gene Beclin 1 and MAP1-LC3 were significantly up-regulated (P<0.01). Conclusion PPD may suppress the proliferation of siha cells,and its mechanism may be related to up-regulating the expressions of autophagy related gene Beclin 1 and MAP1-LC3.

Key words: 20(S)-proto-panaxdiol;siha cell;autophagy;Beclin 1;MAP1-LC3

中图分类号:

许多, 赵丽晶, 程宏, 郭亚雄, 韩向北, 鲁育铭, 赵丽娟, 董妍. 20(S)-原人参二醇对体外培养siha细胞自噬的影响[J]. J4, 2010, 36(6): 1055-1058.

XU Duo, ZHAO Li-Jing, CHENG Hong, GUO Ya-Xiong, HAN Xiang-Bei, LU Yu-Ming, ZHAO Li-Juan, DONG Yan. Effect of 20(S)〖KG-*3〗-proto-panaxdiol on autophag[J]. J4, 2010, 36(6): 1055-1058.

[1]	赵丽晶, 许多, 程宏, 梁作文, 鲁育铭, 韩向北, 郭亚雄, 赵丽娟, 董妍. 20(s)-原人参二醇对体外培养Siha细胞caspase-3表达激活的影响[J]. J4, 2011, 37(1): 26-29.
[2]	赵丽晶, 许多, 梁作文, 程宏, 郭亚雄, 李德隆, 韩向北, 赵丽娟, 董妍. 20(s)-原人参二醇诱导体外培养Siha细胞凋亡的作用[J]. J4, 2010, 36(2): 345-348.