关键词: 骨髓间充质干细胞；胰岛细胞；共培养；糖尿病, 实验性；移植

Abstract:

To observe if the bone marrow mesenchymal stem cells(BMSCs) cocultured with pancreatic cells can be transformed into islet-like cells,and clarify the effect   of blood glucose control  of  BMSCs in diabetic rats. Methods BMSCs and pancreatic cells were  concultured in vitro.18 SD rats were introperitonally injected with streptozotocin(STZ) to set up animal models of diabetes and the rat models were randomly divided into PBS group,BMSCs group and BMSCs cocultured with pancreatic cells group(n=6).The bromodeoxyuridine(Brdu)-labeled cells were transplanted into STZ-inducd diabetic rats by caudal vein,the clinical efficacy was observed.The  distrubition of Brdu-labeled cells  in the pancreas was examined by immunohistochemistry. Results The blood glucose in caudal vein was detected by blood glucose detection instrument.The blood glucose level  in PBS group didn’t change compared with before transplantation(P>0.05).The blood glucose level  was reduced from the 14th day after transplantation in  BMSCs group and BMSCs cocultured with pancreatic cells group,there were significant differences compared with before transplantation (P<0.01).There was  significant difference of the blood glucose level after transplantation between BMSCs group and BMSCs cocultured with pancreatic cells group(P<0.01).The  immunohistochemical results showed that the Brdu-labeled cells with positive  insulin-expression  were tested in the BMSCs group and BMSCs cocultured with pancreatic cells group,but they were  not found in  PBS group. Conclusion The cultured cells could reduce hyperglycemia in the STZ-induced diabetic rats by caudal vein.BMSCs could also reduce hyperglycemia in the STZ-induced diabetic rats by caudal vein.The   effect of the later was better than the former.

Key words: bone marrow mesenchymal stem cells;pancreatic cells;coculture;diabetes,experimental;transplantation