促吸收剂癸酸钠对黄连素在大鼠肠道吸收的促进作用

J4 ›› 2011, Vol. 37 ›› Issue (1): 35-40.

促吸收剂癸酸钠对黄连素在大鼠肠道吸收的促进作用

杜函泽¹, 吕晓艳¹, 杜红伟², 孟昭杰¹, 陈晗³, 陈立¹, 赵珩²

(1.吉林大学白求恩医学院珠江医院眼科|吉林长春 130021；2.吉林大学第一医院干部病房|吉林长春 130021；3. 南方医科大学珠江医院眼科|广东广州 510515)

收稿日期:2010-07-12 出版日期:2011-01-28 发布日期:2011-01-28
通讯作者: 陈立 E-mail:zhouweichen@yahoo.com
作者简介:杜函泽(1987-)|男|吉林省长春市人|医学硕士|主要从事内分泌及肿瘤药理学方面的研究。
基金资助:
吉林省科技厅高技术产业发展项目资助课题（2009633）；吉林大学大学生创新性实验计划项目资助课题(2008A71041)

Absorption enhancement of sodium caprate on berberine in intestine of rats

DU Han-Ze¹, LV Xiao-Yan¹, DU Gong-Wei², MENG Zhao-Jie¹, CHEN Han³, CHEN Li¹, DIAO Hang²

(1. Department of Pharmacology,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China；2.Department of Cadre Ward,First Hospital,Jilin University,Changchun 130021,China；3. Department of Ophthalmology,Pearl River Hospital,Guangzhou 510515,China )

Received:2010-07-12 Online:2011-01-28 Published:2011-01-28

摘要/Abstract

摘要：

目的：探讨黄连素(Ber)在大鼠肠道的吸收动力学，阐明癸酸钠(SC)对Ber在小肠段吸收的影响。方法：雄性Wistar大鼠建立在体肠段灌流模型，HPLC法测定不同浓度Ber（50、100和200 μmol?L-1）和Ber与质量浓度为0.2%SC合用在小肠的吸收情况；测定肠灌流液中乳酸脱氢酶（LDH）活性，观察SC对肠道黏膜细胞的损伤。结果：Ber浓度在50～200 μmol?L-1范围内Ber吸收呈浓度依赖性；吸收速率常数(Ka)组间差异无统计学意义(P>0.05)；SC能显著提高Ber在小肠的吸收量(P<0.05)；SC对Ber吸收速率常数Ka无显著影响（P>0.05）；Ber组、SC＋Ber组肠循环液中LDH的含量与空白对照组比较差异无统计学意义(P>0.05)。结论： Ber在小肠吸收较差，吸收机制为被动扩散，吸收过程为一级动力学过程；SC能显著促进Ber在小肠的吸收，是一种安全、有效的促吸收剂。

关键词: 癸酸钠；黄连素；大鼠, Wistar；小肠；吸收动力学

Abstract:

Abstract:Objective To observe the intestine absorption kinetics of berberine (Ber) in rats and the effects of sodium caprate(SC) on Ber in intestinal absorption. Methods Male Wistar rats were used for the experiment. The in situ circular perfusion model was used to investigate the effect of SC on the intestinal absorption of Ber. The concentration of Ber was detected with HPLC.The activity of LDH was measured to evaluate the toxic effect of SC on mucous membrane cells. Results The absorption of Ber in the small intestine was poor. The uptake of Ber presented dose-dependent manner. There was no significant difference on Ka among these groups (P>0.05). SC could significantly enhance the absorption amount of Ber in the small intestine (P<0.05). SC showed no significant effect on Ka of Ber(P>0.05). The LDH activities in circulating fluid in Ber and SC+Ber groups had no significant difference compared with control group (P>0.05). Conclusion The absorption of Ber in the small intestine is poor and complied with the passive diffusion mechanism and first order kinetics. SC can significantly enhance the absorption of Ber in the small intestine and is a safe,effective absorption accelerator.

Key words: sodium caprate;berberine;rats,Wistar;intestine;absorption kinetics

中图分类号:

R333.3

杜函泽, 吕晓艳, 杜红伟, 孟昭杰, 陈晗, 陈立, 赵珩. 促吸收剂癸酸钠对黄连素在大鼠肠道吸收的促进作用[J]. J4, 2011, 37(1): 35-40.

DU Han-Ze, LV Xiao-Yan, DU Gong-Wei, MENG Zhao-Jie, CHEN Han, CHEN Li, DIAO Hang. Absorption enhancement of sodium caprate on berberine in intestine of rats[J]. J4, 2011, 37(1): 35-40.