短期睡眠剥夺对大鼠外侧缰核内氨基酸类神经递质水平及c-Fos表达的影响

J4 ›› 2011, Vol. 37 ›› Issue (5): 788-791.

短期睡眠剥夺对大鼠外侧缰核内氨基酸类神经递质水平及c-Fos表达的影响

宋美英, 黄民, 王曼丽, 赵华

(吉林大学白求恩医学院生理学系|吉林　长春 130021)

收稿日期:2011-05-09 出版日期:2011-09-28 发布日期:2011-09-28
通讯作者: 赵华 E-mail:（Tel:0431-85619472，E-mail:zhaohua_1957@hotmail.com）
作者简介:宋美英（1974-）,女|吉林省长春市人|在读医学博士|主要从事神经生理学方面的研究。
基金资助:
国家自然科学基金资助课题（30970956）

Effects of sleep deprivation on amino acids neurotransmitters and c-Fos protein expression in lateral habenular nucleus in rats

SONG Mei-Ying, HUANG Min, WANG Man-Li, ZHAO Hua

(Department of Physiology,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China)

Received:2011-05-09 Online:2011-09-28 Published:2011-09-28

摘要/Abstract

摘要：

目的:探讨在短期睡眠剥夺(SD)情况下缰核(Hb)神经元活动的改变和Hb对睡眠的调节作用，阐明外侧缰核在SD后睡眠反弹过程中的作用。方法:将24只健康Wistar大鼠随机分为对照组和SD组，每组各12只。采用单个小平台水环境法制作SD模型，大平台组作为压力对照。高效液相-紫外检测器测量Hb内谷氨酸(Glu)和γ-氨基丁酸(GABA)水平，免疫组织化学方法测定c-Fos阳性神经元的数量。结果:　与对照组比较，SD大鼠Hb内Glu水平明显增加（P<0.05），GABA水平有增加趋势，外侧缰核c-Fos阳性神经元数量增加（P<0.05），内侧缰核c-Fos阳性神经元数量无明显改变。结论:外侧缰核参与短期SD后的睡眠反弹过程，内侧缰核并没有明显作用。

关键词: 睡眠剥夺；外侧缰核；内侧缰核；谷氨酸；γ-氨基丁酸；c-Fos蛋白

Abstract:

Abstract:Objective
To investigate the changes of neuronal activity of habenula and the regulatory effect of Hb on sleep under the condition of short-time sleep deprivation(SD),and to clarify the effects of lateral habenula (LHb) on sleep rebound after short-time SD.Methods 24 adult healthy Wistar rats were randomly divided into control group (n=12) and SD group (n=12).The model of SD was set up by small platform surrounded by water.The typical control for this procedure was to maintain the animal on a large platform.The levels of glutamic acid(Glu) and γ-aminobutyric acid(GABA) in Hb neurons were assessed by HPLC-UV detector.The amounts of c-Fos positive neurons were detected by immunohistochemistry.Results Compared with control group,the levels of Glu in Hb was increased (P<0.05),and the level of GABA had an incrensing trend.The amount of c-Fos positive neurons was increased markedly in LHb(P<0.05),but not in medial Hb(MHb).Conclusion The LHb is related to the sleep rebound after SD,but not the MHb.

Key words: sleep deprivation;lateral habenula;glutamic acid;gamma-aminobutyric acid;c-Fos protein

中图分类号:

R338

宋美英, 黄民, 王曼丽, 赵华. 短期睡眠剥夺对大鼠外侧缰核内氨基酸类神经递质水平及c-Fos表达的影响[J]. J4, 2011, 37(5): 788-791.

SONG Mei-Ying, HUANG Min, WANG Man-Li, ZHAO Hua. Effects of sleep deprivation on amino acids neurotransmitters and c-Fos protein expression in lateral habenular nucleus in rats[J]. J4, 2011, 37(5): 788-791.