喉鳞状细胞癌组织中Galectin-3表达与Bcl-2、Mmp-2及K-ras表达的相关性分析

J4 ›› 2011, Vol. 37 ›› Issue (6): 1116-1119.

喉鳞状细胞癌组织中Galectin-3表达与Bcl-2、Mmp-2及K-ras表达的相关性分析

许燕飞¹|冯青杰¹|滕博¹|赵婕^１|马丽²

1.吉林大学第二医院耳鼻咽喉科|吉林长春 130041；2.内蒙古自治区通辽市中医院耳鼻咽喉科|内蒙古通辽028000

收稿日期:2010-12-09 出版日期:2011-11-28 发布日期:2011-11-28
通讯作者: 冯青杰（Tel：0431-85510109，E-mail：drfeng1960@163.com）；滕博（Tel：0431-85699117，E-mail：tengbo1975@163.com） E-mail:drfeng1960@163.com;tengbo1975@163.com
作者简介:许燕飞(1982-),女,陕西省西安市人|医学硕士|主要从事头颈部肿瘤研究。
基金资助:
吉林省科技厅科研基金资助课题(200305504)

Correlation between expression of Galectin-3 and expressions of Bcl-2,MMP-2 and K-ras in laryngeal squamous carcinoma tissue

XU Yan-fei¹,FENG Qing-jie¹,TENG Bo¹,ZHAO Jie¹|MA Li²

1.Department of Otolaryngology,Second Hospital,Jilin University,Changchun 130041,China；2.Department of Otolaryngology,Tongliao Traditional Chinese Medical Hospital,Tongliao 028000,China

Received:2010-12-09 Online:2011-11-28 Published:2011-11-28

摘要/Abstract

摘要：

目的：研究半乳糖凝集素-3（Galectin-3）在喉鳞状细胞癌(LSCC)组织中的表达与临床病理特征的关系，并探讨其与Bcl-2、Mmp-2及K-ras表达的相关性。方法：采用SP免疫组织化学法检测Galectin-3、Bcl-2、Mmp-2及K-ras在32例喉鳞状细胞癌组织、32例喉正常组织及10例声带息肉组织标本中的表达，分析其与喉鳞状细胞癌病理学特征的关系，以及Galectin-3表达与Bcl-2、Mmp-2及K-ras表达的相关性。结果：喉正常组织、声带息肉组织及喉癌组织3组间Galectin-3、Bcl-2、Mmp-2及K-ras的表达比较差异有统计学意义（P<0.05）。Galectin-3和Bcl-2的表达与喉癌病理分级相关（P<0.05），Bcl-2的表达与喉癌的临床分期、淋巴结转移密切相关（P<0.05）。Galectin-3在喉癌组织中的表达与Bcl-2、Mmp-2及K-ras在喉癌组织中的表达呈正相关（r₁＝0.993，r_２＝0.989， r３＝0.992，P<0.05）。结论： Galectin-3、Bcl-2、Mmp-2及K-ras的高表达与喉癌的发生密切相关； Galectin-3表达与Bcl-2、Mmp-2及K-ras的表达呈正相关，在喉癌的发生和发展中起重要作用，有望成为喉癌基因治疗的新的靶基因。

关键词: 喉肿瘤；Galectin-3；Bcl-2；Mmp-2；K-ras；免疫组织化学

Abstract:

Objective To study the correlation between the expression of Galectin-3 and Bcl-2,Mmp-2 and K-ras in laryngeal squamous carcinoma(LSCC) and the relationship between Galetin-3 expression and clinicopathological characteristics.Methods The expressions of Galectin-3,Bcl-2,Mmp-2 and K-ras in LSCC tissues of 32 patients,normal mucosa tissues of 32 cases,and polyp of vocal cord tissues of 10 cases were detected by immunohistochemistry staining.The relationship between their expressions and the pathological characteristics of LSCC,clinical staging and lymph node metastasis in LSCC was analyzed.The correlation between the expression of Galectin-3 in LSCC tissue and Bcl-2,Mmp-2 and K-ras expressions in LSCC tissue was analyzed.Results The differences in the expressions of Galectin-3,Bcl-2,Mmp-2 and K-ras between normal mucosa tissues,polyp of vocal cord tissues and LSCC tissues were significant （P<0.05）.The expressions of Galectin-3 and Bcl-2 were related to pathological grade of LSCC（P<0.05）,the expression of Bcl-2 was related to clinical stages and lymph node metastasis of LSCC（P<0.05）.The expression of Galectin-3 had positive correlation with Bcl-2,Mmp-2, and K-ras expressions in LSCC tissue(r₁＝0.993，r_２＝0.989，r_３＝0.992).Conclusion The high expressions of Galectin-3,Mmp-2 and K-ras are closely related to the occurrence and development of the LSCC.The expression of Galectin-3 is positively related to the expressions of Bcl-2,Mmp-2 and K-ras.Co-expression of Galectin-3,Bcl-2,Mmp-2 and K-ras are able to promote the pathogenesis of LSCC.Galectin-3 is possible to become a new target gene of LSCC gene theray.

Key words: laryngeal neoplasm, Galectin-3, Bcl-2, Mmp-2, K-ras； immunohistochemistry

中图分类号:

R739.65

许燕飞|冯青杰|滕博|赵婕|马丽. 喉鳞状细胞癌组织中Galectin-3表达与Bcl-2、Mmp-2及K-ras表达的相关性分析[J]. J4, 2011, 37(6): 1116-1119.

XU Yan-fei,FENG Qing-jie,TENG Bo,ZHAO Jie|MA Li. Correlation between expression of Galectin-3 and expressions of Bcl-2,MMP-2 and K-ras in laryngeal squamous carcinoma tissue[J]. J4, 2011, 37(6): 1116-1119.

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