J4 ›› 2012, Vol. 38 ›› Issue (3): 393-398.

• 基础研究 •    下一篇

电离辐射对成纤维细胞中透明质酸蛋白及透明质酸酶1 mRNA表达水平的影响

刘姗姗1|高瀚男2|张连波3|宋文刚4|孙颖1|孙世龙5|高庆国3   

  1. (1.吉林大学公共卫生学院营养与食品卫生学教研室|吉林 |长春 130021；2.吉林大学生命科学院|吉林 长春 130012；3.吉林大学中日联谊医院显微整形外科,吉林 长春 130033；4.北华大学基础医学院微生物学教研室|吉林 吉林 132013 ；5.吉林大学公共卫生学院 卫生部放射生物学重点实验室|吉林 长春 130021)
  • 收稿日期:2011-12-01 出版日期:2012-05-28 发布日期:2012-05-28
  • 通讯作者: 高庆国 E-mail:（Tel：0431-85619433，E-mail：gaoqingguo2008@yahoo.cn）
  • 作者简介:刘姗姗（1986-）|女|吉林省蛟河市人| 在读医学硕士,主要从事营养基因组学研究。
  • 基金资助:

    国家自然科学基金资助课题（30973057）；          吉林省人才开发基金资助课题（802090000430）；          吉林省科技厅科技发展计划项目资助课题（200705269）；          吉林大学基本科研业务费项目资助课题（200903117）;吉林省科学技术教育厅“十二五”科学技术研究项目资助课题［吉教科合字（2011）第154号］

Effect of ionizing radiation on expression levels of hyaluronic acid protein and |HAase1 mRNA in human fibroblasts

LIU Shan-shan1,GAO Han-nan2,ZHANG Lian-bo3,SONG Wen-gang4,SUN Ying1,SUN Shi-long5,GAO Qing-guo3   

  1. (1. Department of Nutrition and Food Hygiene, Shcool of Public Health,Jilin University,Changchun 130021,China;2. Shcool of Life Sciences,Jilin University,Changchun 130012,China;3. Department of Microscopic Plastic Surgery,China-Japan Union Hospital,Jilin University,Changchun 130033,China;4. Department of　Microbiology| School of Basic Medical Sciences,Beihua |University,Jilin 132013,China;5. Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China)
  • Received:2011-12-01 Online:2012-05-28 Published:2012-05-28

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摘要：

目的:观察电离辐射对成纤维细胞中透明质酸（HA）蛋白及透明质酸酶1（HAase1） mRNA表达水平的影响，并探讨其在放射性臂丛神经损伤发病机制中的作用。方法: 成纤维细胞分别采用0.5、1.0、2.0、4.0和6.0 Gy X射线照射，作为 5个剂量组，同时设假照射对照组（0 Gy）。透射电镜观察0、2.0、4.0和6.0 Gy X射线照射后48 h成纤维细胞的形态变化。采用ELISA法和RT-PCR法检测0、0.5、1.0、2.0、4.0、和6.0 Gy X射线照射后24及48 h成纤维细胞中HA蛋白及HAase1 mRNA表达水平的变化。结果：2.0 Gy X射线照射后48 h细胞染色质凝聚，有空泡形成；4.0 Gy X射线照射后48 h细胞核凹陷、染色质断裂；6.0 Gy X射线照射后细胞核凹陷、染色质固缩、出现空泡，部分胞浆溶解。不同剂量X射线照射后24 h HA蛋白表达均明显增高，与0 Gy组比较差异有统计学意义(P<0.05)；0.5～2.0 Gy X射线照射后48 h HA蛋白表达变化不明显,4.0和6.0 Gy组与 0 Gy组比较，差异有统计学意义（P<0.05）。0～6.0 Gy X射线照射后24  h HAase1 mRNA水平明显增高，与0 Gy组比较，差异有统计学意义（P<0.05）。1.0、4.0和6.0 Gy X射线照射后48 h HAase1 mRNA水平明显增高，与0 Gy组比较，差异有统计学意义(P<0.05)。结论：电离辐射可诱导人成纤维细胞中HA蛋白表达和HAase1 mRNA水平增高，可能对放射性臂丛神经损伤的发生发展起到一定的作用。

关键词: 辐射, 电离;成纤维细胞;透明质酸;透明质酸酶1

Abstract:

To investigate the effect of ionizing radiation on the expression levels of hyaluronic acid(HA) protein and hyaluronidase(HAase1) mRNA in human fibroblasts and to discuss the role of HA protein and HAase1 mRNA in the pathogenic mechanism of radiation-induced brachial plexus injury.Methods The human fibroblasts were divided into   0.5,1.0,2.0,4.0 and 6.0 Gy X-rays radiation groups and sham irradiation group (0 Gy). The morphological changes of the fiber cells after irradiated by different doses of X-rays were observed.The expression levels of HA protein and  HAase1 mRNA at 24  and 48 h after irradiated by different doses of 0,0.5,1.0,2.0,4.0 and 6.0 Gy X-rays were detected by ELISA and RT-PCR methods.Results The cells appeared vacuolization，and the nuclear chromatic began to get together after 2.0 Gy exposure;the nuclear saged and  the chromatin fractured after 4.0 Gy exposure;the nuclear saged,chromatin fractured and the part of cell plasma was dissolved after 6.0 Gy exposure.Compared with 0 Gy group,the expressions of HA protein were  increased significantly at 24 h after X-rays irradiation with the doses of 0.5,1.0,2.0,4.0 and 6.0 Gy(P<0.05) and the expression of HA protein had no  significant change  at 48 h after irradiation,but the expressions of HA protein in 4.0 and 6.0 Gy groups were increased significantly  compared with 0 Gy group (P<0.05). The levels of HAase1 mRNA were markedly increased at 24 h  after irradiated by different doses of X-rays (P<0.05).Compared with 0 Gy group,the expressions of HAase1 mRNA were increased significantly at 48 h after 1.0，4.0 and 6.0 Gy exposure compared with 0 Gy group(P<0.05).Conclusion The X-rays irradiation can induce  the increase of the expression of HA protein and the level of HAase1 mRNA in human fibroblasts ,and it may influence the occurrence and development of radiation-induced brachial plexus injury.

Key words: radiation,ionizing;fibroblasts;hyaluronic acid；hyaluronidase

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  • R147 
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