J4 ›› 2012, Vol. 38 ›› Issue (5): 961-964.

• 基础研究 • 上一篇    下一篇

中药马勃对大鼠皮肤成纤维细胞增殖及胶原合成的影响

石毅1|刘忠英2|卢泽源*|刘迪1|刘彬彬Δ,周余来1|刘志3   

  1. 1. 吉林大学药学院生物工程实验中心|吉林 长春 130021;2. 吉林大学药学院药物分析教研室|吉林 长春 130021;3. 吉林大学数学学院|吉林 长春 130012
  • 收稿日期:2012-04-11 出版日期:2012-09-28 发布日期:2012-09-28
  • 通讯作者: 刘 志(Tel:0431-85166967,E-mail:liuzhi@jlu.edu.cn) E-mail:liuzhi@jlu.edu.cn
  • 作者简介:石 毅(1962-)|女|吉林省长春市人|研究员|主要从事中药生物工程的研究。
  • 基金资助:

    吉林省中医药管理局资助课题 (2010-pt063)

Effects of traditional puffball on proliferation and collagen synthesis of skin fibroblasts in rats

SHI Yi1|LIU Zhong-ying2|LU Ze-yuan*|LIU Di1|LIU Bin-binΔ|ZHOU Yu-lai1|LIU Zhi3   

  1. 1.Center for Biological Engineering Experiment|School of Pharmacy|Jilin University|Changchun 130021|China;2. Department of Drug Analysis|School of Pharmacy|Jilin University|Changchun 130021|China;3. School of Mathematics|Jilin University|Changchun 130012|China
  • Received:2012-04-11 Online:2012-09-28 Published:2012-09-28

摘要:

[摘 要] 目的:中药马勃作用于体外培养大鼠皮肤的成纤维细胞,观察大鼠成纤维细胞增殖及胶原合成的能力,探讨马勃有效组分在细胞水平上促皮肤患处愈合的作用机制。方法:运用体外细胞培养技术对新生大鼠乳鼠皮肤的成纤维细胞进行培养,实验分为对照组(不给药组)和马勃有效组分37.5、75.0、150.0及300.0 mg/L组;实验组于传代培养24 h后加入不同浓度的药物,继续培养24、48、72 h后收集细胞。采用MTT比色法测定细胞增殖,生物素双抗体夹心酶联免疫吸附法测定细胞胶原含量。结果:与对照组比较,37.5、75.0、150.0及300.0 mg/L实验组的成纤维细胞增长率和成纤维细胞胶原合成能力均显著性升高(P<0.01)。在药物作用时间方面,与对照组比较,在24、48和72 h观察细胞增殖率和胶原合成能力均显著增强,72 h最强,差异有统计学意义(P<0.01)。结论:在不同剂量马勃有效组分作用下,大鼠成纤维细胞活化增殖,胶原合成的能力增强。

关键词: 马勃;成纤维细胞;细胞增殖;胶原合成

Abstract:

Abstract:Objective To study the effects of puffball on proliferation and collagen synthesis on rat skin fibroblasts, and to investigate the  mechanism of  enhancing wound healing  of  active constituent of puffball     at the cells level.Methods The rat skin fibroblasts were divided into 5 groups, all the cells were treated with puffball(37.5,75.0,150.0 and 300.0 mg/L), except for control group,for 24,48 and 72 h,respectively.And then MTT was used to detect the proliferation, and the content of collagen were measured by double antibody sandwich ELISA.Results  Compared with control group, the cells treated with different concentrations (37.5,75.0,150.0 and300.0 mg/L ) of puffball showed a significant rise both in its proliferation and the ability of collagen synthesis (P<0.01).Furthermore, in time-dependant manner,  the proliferation rate of cells and collagen synthesis ability  wer  significantly increased after treated for 24,48,72 h,especially in 72 h(P<0.01). Conclusion  Puffball with different doses  can enhance the proliferation and collagen synthesis in  skin fibroblasts of rats.

Key words: puffball;fibroblast;cell proliferation;collagen synthesis

中图分类号: 

  • R965