实时荧光定量PCR法检测EG-1在甲状腺乳头状癌组织中的表达及其临床意义

J4 ›› 2012, Vol. 38 ›› Issue (5): 981-984.

实时荧光定量PCR法检测EG-1在甲状腺乳头状癌组织中的表达及其临床意义

孙亚东|张景斌|杨乐|马彦|姜春浩| 邸军

吉林省人民医院内分泌一疗区,吉林长春 130021

收稿日期:2012-04-10 出版日期:2012-09-28 发布日期:2012-09-28
通讯作者: 孙亚东(Tel:0431-85595185,E-mail：ydsun-2005@163.com) E-mail:ydsun-2005@163.com
作者简介:孙亚东（1964-）|女|吉林省长春市人|主任医师|医学博士|主要从事内分泌代谢病基础及临床研究。
基金资助:
吉林省科技厅自然科学基金资助课题(201015127)

Expression |of EG-1 in human thyroid papillary carcinoma tissue detected by real-time quantitative 
PCR and its clinical significance

SUN Ya-dong, ZHANG Jing-bin, YANG Le,MA Yan, |JIANG Chun-hao,DI |Jun

Department of Endocrinology|People’s Hospital of Jilin Province, Changchun 130021,China

Received:2012-04-10 Online:2012-09-28 Published:2012-09-28

摘要/Abstract

摘要：

目的：通过实时荧光定量PCR法检测内皮衍生基因-1(EG-1)的基因在甲状腺癌组织中的表达,探讨EG-1与甲状腺癌发生、发展的关系。方法：选取38例甲状腺乳头状癌患者（病例组）和15例良性甲状腺疾病患者的甲状腺组织(对照组）,采用实时荧光定量PCR法检测甲状腺组织中EG-1基因的表达，同时应用免疫组化学法检测EG-1的表达水平。结果：免疫组织化学结果显示, EG-1主要定位于癌细胞的细胞质，甲状腺乳头状癌组织中EG-1 mRNA和蛋白表达水平明显高于良性甲状腺疾病患者 (P<0.05)。EG-1 mRNA表达与组织学分级有关(P<0.05)，与性别、年龄无明显关联性(
P>0.05)。结论：EG-1基因在甲状腺乳头状癌组织中表达明显增高，EG-1基因可能在甲状腺癌的发生、发展中起重要作用。

关键词: 实时荧光定量PCR, 内皮衍生基因-1；甲状腺肿瘤

Abstract:

Objective To detect the expression of endothelial derived gene-1 (EG-1) gene in thyroid papillary carcinoma tissue by real-time quantitative PCR and to investigate the relationship between EG-1 and the ocurrence and development of thyroid papillary carcinoma. Methods Real-time quantitative PCR was used to detect
the expressions of EG-1 gene in 38 thyroid papillary carcinoma patients ( case group) and 15 thyroid adenoma patients (control group),and the expression level of EG-1 was detected by immunohistochemisty.
Results The expressions of EG-1 mRNA and protein in thyroid papillary carcinoma tissue were significantly higher than those in adenoma tissue（P<0.05）.The expression of EG-1 mRNA had significant relationship with the pathological degree in thyroid cancer(P<0.05), but there was no correlation with the age and the gender of the patients(P>0.05).Conclusion The expression of EG-1 gene in thyroid papillary carcinoma tissue is obviously higher,EG-1 gene may be important in the occurrence and development of thyroid cancer.


Key words: real-time quantitative polymerase chain reaction, endothelial derived gene-1, thyroid neoplasms

中图分类号:

R736.1

孙亚东|张景斌|杨乐|马彦|姜春浩| 邸军 . 实时荧光定量PCR法检测EG-1在甲状腺乳头状癌组织中的表达及其临床意义[J]. J4, 2012, 38(5): 981-984.

SUN Ya-dong, ZHANG Jing-bin, YANG Le,MA Yan, JIANG Chun-hao,DI Jun . Expression |of EG-1 in human thyroid papillary carcinoma tissue detected by real-time quantitative 
PCR and its clinical significance[J]. J4, 2012, 38(5): 981-984.

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