吉林大学学报(医学版)

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乳腺癌组织中神经营养因子Neuritin的表达及其临床意义

曹 健1,王 瑞2,侯 进3   

  1. 1.西安医学院生理学教研室,陕西 西安 710021;2.西安交通大学医学院第一附属医院
    中医科,陕西 西安 710061;3. 西安医学院药理学教研室,陕西 西安 710021
  • 收稿日期:2012-12-09 出版日期:2013-07-28 发布日期:2013-08-17
  • 通讯作者: 侯 进(Tel:029-86177562,E-mail: houandjin@hotmail.com) E-mail:houandjin@hotmail.com
  • 作者简介:曹 健(1975-),男,陕西省西安市人,讲师,医学硕士,主要从事帕 金森病发病机制的研究。
  • 基金资助:

    陕西省教育厅科研基金资助课题(2010JK805);西安医学院校级基金资助
    课题(12FZ14)

Expression of neuritinrotrophic factor Neuritin in breast 
cancer tissue and its clinical significance

CAO Jian1,WANG Rui2,HOU Jin3   

  1. 1.Department of Physiology,Xi’an Medial College,Xi’an 710021,China;2.
    Department of Traditional Chinese Medicine,First Affiliated Hospital,Medical College,Xi’an
     Jiaotong University,Xi’an 710061,China;3.Department of Pharmacology,Xi’an Medial College,Xi’an 710021,China
  • Received:2012-12-09 Online:2013-07-28 Published:2013-08-17

摘要:

目的:观察神经营养因子Neuritin蛋白和mRNA在人乳腺癌组织中的表达及其
与临床病理特征的关系,探讨其在乳腺癌发生发展中的作用。方法:  收集74例乳腺癌组织
标本和12例正常乳腺组织标本,采用免疫组织化学和Western blotting法检测标本中Neuri
tin蛋白的阳性表达率和表达水平,利用RT-PCR法检测标本中Neuritin mRNA的表达水平。结
果:Neuritin蛋白在正常乳腺组织中阳性表达率为25%,在乳腺癌组织中阳性表达率为70.62%,
乳腺癌组织中Neuritin蛋白阳性表达率高于正常乳腺组织(P<0.05),且其在乳腺癌肿瘤细胞中定位于细胞核和细胞质。Neuritin蛋白的阳性表达率与乳腺癌淋巴
结转移、TNM分期和ER/PR/HER2高阳性率有关联(P<0.05)。与正常乳腺组织比较,Ⅰ-Ⅱ
期及Ⅲ-Ⅳ期乳腺癌组织中Neuritin蛋白和mRNA表达水平升高(P<0.05,P<0.01);与Ⅰ
-Ⅱ期乳腺癌组织比较,Ⅲ-Ⅳ期乳腺癌组织中Neuritin蛋白和mRNA表达水平亦升高(P<0.05)。结
论:Neuritin可能是促进乳腺癌发生发展的一个重要基因,也可能是治疗乳腺癌的关键靶向分子。
 

关键词: 乳腺肿瘤, 神经生长因子, Neuritin, 实时荧光定量PCR法

Abstract:

Abstract:Objective To observe the expressions of neuritinrotrophic factor
 Neuritin protein and mRNA in human breast cancer tissue and the relationship between
 its expression and the clinicopathological features,and to explore its roles
 in the carcinogenesis and progression of breast cancer.Methods
74 cases of breast cancer tissue samples and 12 cases of normal breast tissue samples were coll
ected.The expressions of Neuritin protein in samples were detected with immunohistochemical assay and Western blotting method;the expression levels of Neuritin mRNA  in samples were detected by quantitative  RT-PCR.
Results The expression rate of Neuritin  in normal breast tissue was 25%,
and the positive expression rate of Neuritin in breast cancer tissue  was 70.62%,which was higher than that in norma
l breast tissue (P<0.05);Neuritin mainly located in the nucleus and cytoplasm.Meantime,there were positive correlations between the positive expression of Neuritin and lymph node metastasis,TNM stages and the positive rate of ER/PR/HER2.Compared with normal breast tissue,the expression levels of Neuritin protein and mRNA in stage Ⅰ-Ⅱ and Ⅲ-Ⅳ breast cancer  tissue were increased (P<0.05 or P<0.01);compared with stage Ⅰ-Ⅱ breast cancer tissue,the expresson leve
ls of Neuritin protein and mRNA in stage Ⅲ-Ⅳ breast cancer tissue was significantly increased(P<0.05).Conclusion Neuritin may be one
 of the genes to enhance the growth of breast cancer and may be a target for the treatment of breast cancer.

Key words: breast neoplasms, neuritinrotrophic factor, Neuritin, quantitative fluorescence real-time PCR

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