吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (2): 268-272.doi: 10.7694/jldxyxb20130217

• 基础研究 • 上一篇    下一篇

丹酚酸B对心肌梗死模型大鼠心肌的保护作用及其分子机制

张强1,杨丽红2,李涵   

  1. 1.郑州大学第二附属医院心血管内科,河南 郑州 450014;2. 郑州大学第二附属医院心电图科,河南 郑州 450014
  • 收稿日期:2012-09-12 出版日期:2013-03-28 发布日期:2013-03-26
  • 通讯作者: 张强(Tel:0371-65705016,E-mail:zq3397@163.com) E-mail:zq3397@163.com
  • 作者简介:张 强(1971-),男,河南省郑州市人,教授,主任医师,医学硕士,主要从事心血管系统疾病基础与临床研究。
  • 基金资助:

    河南省科技厅科研基金资助课题(72102310023)

Protective effect  of salvianolic acid B  on myocardium of   myocardial infarction model rats  and its molecular mechanism

ZHANG Qiang1,YANG Li-hong2,LI Han1   

  1. 1.Department of Cardiology,Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014,China;2.Department of Electrocardiogram,Second Affiliated Hospital,Zhengzhou University, Zhengzhou 450014,China
  • Received:2012-09-12 Online:2013-03-28 Published:2013-03-26

摘要: 目的:观察丹酚酸B(Sal B)对心肌梗死模型大鼠心肌的保护作用,阐明Sal B干预大鼠心肌梗死的分子机制。方法:将大鼠随机分为对照组、模型组、阳性药实验组(AsA组和BHT组)和观察药实验组(Sal B组),采用冠状动脉结扎法构建大鼠心肌梗死模型。各实验组大鼠均按120 mg/kg剂量腹腔注射给药,模型组与对照组大鼠注射相同剂量的蒸馏水,连续7 d。测定各组大鼠血清中肌酸磷酸激酶(CK)、天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)的活性,并利用ELISA法分析各组大鼠血清中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的表达水平;流式细胞术检测各组大鼠心肌细胞凋亡率,分光光度法检测自由基清除率。结果:与对照组比较,模型组大鼠血清CK、AST和LDH活性明显升高(P<0.01);与模型组比较,阳性药实验组和Sal B组大鼠血清CK、AST和LDH活性明显降低(P<0.05或P<0.01)。与对照组比较,模型组大鼠血清中IL-1β、IL-6、TNF-α水平明显增加(P<0.01);与模型组比较,Sal B组大鼠血清中IL-1β、IL-6和TNF-α水平显著降低(P<0.01);而AsA和BHT组上述3种因子水平无明显变化(P>0.05)。与对照组比较,模型组大鼠心肌细胞凋亡率明显增加(P<0.01);与模型组比较,Sal B组大鼠心肌细胞凋亡率明显降低(P<0.01);而AsA和BHT组大鼠心肌细胞凋亡率无明显改变(P>0.05)。不同浓度的Sal B均能提高1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除率(P<0.05),且20~100 mg/L Sal B对自由基的清除率均高于AsA和BHT(P<0.05或P<0.01)。结论:Sal B对大鼠冠状动脉结扎诱发实验性心肌梗死具有一定的保护作用,可以有效抑制心肌细胞凋亡,其作用机制可能与抑制免疫炎症因子的分泌和自由基清除作用有关。

关键词: 丹酚酸B, 心肌梗死, 细胞凋亡, 自由基, 炎症因子

Abstract: Abstract:Objective To investigate the protective effect   of salvianolic acid B  (Sal B) on myocardium of   myocardial infarction model rats and to clarify the molecular mechanism of Sal B intervention in myocardial infarction.Methods The  SD rats were randomly divided into control group,model group,positive drug experiment (AsA and BHT)  groups and observation drug experiment group(Sal B group).The myocardial infarction models of  rats were set up by  coronary artery ligation.The rats in every experiment groups were administered by intraperitoneal injection with the dose of 120 mg?kg-1,the rats in  model group and control group were injected  with the same dose of distilled water for 7 d.Then  the activities of CK,AST and LDH in serum were detected,and  ELISA assay  was used to analyze the expression levels of IL-1β,IL-6 and TNF-α in serum;and  the apoptotic rate  of cardiocytes  in each group was measured,and the clearance rate of free radical was determined by absorption spectrometry.Results  Compared with control group,the activities of serum CK,AST and LDH in model group were increased   obviously(P<0.01);compared with model group,the activities of serum CK,AST and LDH  in positive drug and Sal B groups were decreased obviously(P<0.05 or P<0.01).Compared with control group,the levels of serum IL-1β,IL-6 and TNF-α  in model group were increased  obviously(P<0.01);compared with model group,the levels of serum IL-1β,IL-6 and TNF-α in Sal B group were decreased significantly (P<0.01);but there was no marked change in AsA and BHT groups(P>0.05).Compared with control group,the apoptotic rate  of cardiocytes  in model group was increased obviously(P<0.01);compared with model group,the apoptotic rate of cardiocytes was decreased significantly  in Sal B group (P<0.01);there was no marked change in AsA and BHT groups(P>0.05).Different concentrations of Sal B  increased the clearance rate of DPPH free radical  with statistical difference (P<0.05).The clearance rates of free radical in Sal B group were higher than those in AsA and BHT groups when the concentrations of Sal B were 20-100 mg/L(P<0.05 or P<0.01).Conclusion Sal B has a protective effect on    experimental myocardial infarction   in rats,and  effectively inhibit apoptosis  of cardiocytes.Its mechanism may be related to suppression of immune and inflammatory factor secretion and  free radical scavenging.

Key words: salvianolic acid B, myocardial infarction, apoptosis, free radical, inflammatory factor

中图分类号: 

  • R542.22